Aluminum-mediated metabolic changes in rat serum and urine: A proton nuclear magnetic resonance study

Sandeep Tripathi, B. S. Somashekar, Abbas Ali Mahdi, Ashish Gupta, Farzana Mahdi, Mahdi Hasan, Raja Roy, C. L. Khetrapal

Research output: Contribution to journalArticle

Abstract

The toxic effects of Al3+ have been studied in 90-days AlCl 3 orally treated male albino rats (n = 7) using 1H NMR spectroscopy-based metabolic profile of rat serum and urine, serum enzyme tests, behavioral impairment, and histopathology of kidney and liver. Metabolic profile of 90-days Al3+-treated rat sera showed significantly elevated levels of alanine, glutamine, β-hydroxy-butyrate, and acetoacetate and significantly decreased level of acetone when compared with that of control rats. However, metabolic profile of 90-days Al3+-treated rat urine showed significantly decreased levels of citrate, creatinine, allantoin, trans-aconitate, and succinate and significantly increased level of acetate when compared to control rats. The overall perturbations observed in the metabolic profile of serum and urine demonstrate the impairment in the tricarboxylic acid cycle, liver and kidney metabolism, which was further reinstated by clinical chemistry and histopathological observations. Moreover, "in vivo" behavioral impairment has also been observed as the indication of aluminum neurotoxicity.

Original languageEnglish (US)
Pages (from-to)119-127
Number of pages9
JournalJournal of Biochemical and Molecular Toxicology
Volume22
Issue number2
DOIs
StatePublished - May 15 2008

Keywords

  • Al toxicity
  • H NMR
  • Metabolic alterations
  • Rat serum
  • Rat urine
  • TCA cycle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Toxicology
  • Health, Toxicology and Mutagenesis

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  • Cite this

    Tripathi, S., Somashekar, B. S., Mahdi, A. A., Gupta, A., Mahdi, F., Hasan, M., Roy, R., & Khetrapal, C. L. (2008). Aluminum-mediated metabolic changes in rat serum and urine: A proton nuclear magnetic resonance study. Journal of Biochemical and Molecular Toxicology, 22(2), 119-127. https://doi.org/10.1002/jbt.20219