Alu-PCR: Characterization of a chromosome 6-specific hybrid mapping panel and cloning of chromosome-specific markers

Eckart U. Meese, Paul S. Meltzer, Paul W. Ferguson, Jeffrey M. Trent

Research output: Contribution to journalArticle

Abstract

The Alu-polymerase chain reaction (Alu-PCR) was applied to selectively amplify DNA sequences from human chromosome 6 using a single primer (A1) directed to the human Alu consensus sequence. A specific amplification pattern was demonstrated for a panel of eight somatic cell hybrids containing different portions of chromosome 6. This PCR pattern permits the identification of submicroscopic DNA alterations and can be utilized as a reference for additional chromosome 6-specific hybrids. To obtain new chromosome 6-specific markers we established two libraries from PCR-amplified sequences using two somatic cell hybrids (MCH381.2D and 640-5A). Out of a total of 109 clones that were found to be chromosome 6 specific, 13 clones were regionally assigned. We also included a procedure that allows the isolation of chromosome 6-specific markers from hybrids that contain human chromosomes other than 6. Our results will contribute to the molecular characterization of chromosome 6 by fostering characterization of somatic cell hybrids and by the generation of new regicnally assigned DNA markers.

Original languageEnglish (US)
Pages (from-to)549-554
Number of pages6
JournalGenomics
Volume12
Issue number3
DOIs
StatePublished - Mar 1992

ASJC Scopus subject areas

  • Genetics

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