Alternative splice forms of the murine t-cell receptor β transcript in a cytotoxic t-cell line

Dale G. Schaar, Bermseok Oh, Laura M. Kallay, Paul J. Doherty, Sueihua Pan

Research output: Contribution to journalArticle

Abstract

The processing of the primary transcript of the murine T cell receptor Cβ region can result in two distinct forms of protein. The optional exon, CβO, is appended at the 5' end of the constant region and to date has been observed at low frequencies in cDNA clones from heterogeneous populations of T lymphocyte. Because individual cell lines were not analyzed in those studies, it was not known whether small numbers of T lymphocytes use the CβO exon exclusively or instead most T-lymphocytes use the CβO exon but at low levels. We have determined that our CTL clone, B6.c1 4, produces both CβO+ and CβO- functional RNA transcripts. To determine the CβO usage in this CTL clone, we coupled cDNA synthesis with the polymerase chain reaction using oligonucleotides corresponding to sequences at the Vβ14 region and sequences corresponding to CβO or Cβ1. The data indicate that B6.c1 4 CTL clone is able to use either splice site but uses the CβO exon at a frequency of 2-3%

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalImmunological Investigations
Volume19
Issue number2
DOIs
Publication statusPublished - 1990
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

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