Alternative mRNA splice variants of the rat ClC-2 chloride channel gene are expressed in lung: Genomic sequence and organization of ClC-2

Shijian Chu, Pamela L. Zeitlin

Research output: Contribution to journalArticlepeer-review

Abstract

The ClC-2 epithelial cell chloride channel is a voltage-, tonicity- and pH-regulated member of the ClC super family. We have previously shown that rat lung ClC-2 (rClC-2) is down-regulated at birth, and molecular diversity is generated by alternative splicing. To investigate other possible mRNA splice variations, we sequenced the entire rClC-2 gene and found that ClC-2Sa (formerly ClC-2S) results from the deletion of exon 20. The preceding intron 19 has an unusually high CT content and a rare AAG acceptor site. Because both features were also found in intron 13, we next tested the hypothesis that intron 13 would be involved in alternative splicing. As predicted, a second splice product, ClC-2Sb, was found by RT-PCR, but only in lung. When we compared the genomic maps of rClC-2 and human ClC-1 (hClC-1), striking similarities were found in each exon except for rClC-2 exon 20, which is absent in hClC-1. These observations suggest that ClC-1 and ClC-2 may have evolved by gene duplication, mutation and DNA rearrangement.

Original languageEnglish (US)
Pages (from-to)4153-4159
Number of pages7
JournalNucleic acids research
Volume25
Issue number20
DOIs
StatePublished - Oct 15 1997

ASJC Scopus subject areas

  • Genetics

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