Altered subcellular localization of the NeuN/Rbfox3 RNA splicing factor in HIV-associated neurocognitive disorders (HAND)

Calixto Hope Lucas, Mathilde Calvez, Roshni Babu, Amanda Brown

Research output: Contribution to journalArticle

Abstract

The anti-NeuN antibody has been widely used for over 15 years to unambiguously identify post-mitotic neurons in the central nervous system of a wide variety of vertebrates including mice, rats and humans. In contrast to its widely reported nuclear localization, we found significantly higher NeuN reactivity in the cytoplasm of neurons in brain sections from HIV-infected individuals with cognitive impairment compared to controls. The protein target of anti-NeuN antisera was recently identified as the neuron-specific RNA splicing factor, Rbfox3, but its significance in diseases affecting the brain has not been previously reported. RNA splicing occurs in the nucleus hence, the altered localization of RbFox3 to the cytoplasm may lead to the downregulation of neuronal gene expression.

Original languageEnglish (US)
Pages (from-to)97-102
Number of pages6
JournalNeuroscience Letters
Volume558
DOIs
StatePublished - Jan 13 2014

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Keywords

  • Gene expression
  • HIV-associated neurocognitive disorders
  • NeuN
  • Neurodegeneration
  • Rbfox3
  • Splicing

ASJC Scopus subject areas

  • Neuroscience(all)

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