Altered L-type Ca2+ channel currents in vascular smooth muscle cells from experimental diabetic rats

Rui Wang, Yuejin Wu, Guanghua Tang, Lingyun Wu, Salma Toma Hanna

Research output: Contribution to journalArticle

Abstract

Vascular complications of diabetes are associated with abnormal Ca2+ handling by vascular smooth muscle cells (SMCs) in which the alteration in L- type voltage-dependent Ca2+ channel (VDCC) currents may play an important role. In the present study, the characteristics of L-type VDCC currents in tail artery SMCs from streptozotocininduced diabetic rats were examined. The densities, but not the voltage dependence, of L-type VDCC currents were reduced as diabetes progressed from 1 wk to 3 mo. The inhibitory effect of dibutyryl-cAMP on L-type VDCC currents was greater in diabetic SMCs than in age-matched control cells (P < 0.01). Both the stimulatory effect of BAY K 8644 and the inhibitory effect of nifedipine on L-type VDCC currents were significantly enhanced in diabetic cells. The diabetes-related abnormalities in L-type VDCC currents were mimicked by culturing SMCs with a high concentration of glucose. Our results suggest that the properties of L-type VDCC in diabetic vascular SMCs were significantly altered, partially related to the increased L-type VDCC sensitivity to cAMP and hyperglycemia.

Original languageEnglish (US)
Pages (from-to)H714-H722
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume278
Issue number3 47-3
DOIs
StatePublished - Mar 2000

Keywords

  • Artery
  • Calcium channels
  • Dihydropyridines
  • Hyperglycemia
  • Patch-clamp

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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