Altered glycoprotein synthesis in mouse epidermal cells treated with retinyl acetate in vitro

L. DeLuca, S. H. Yuspa

Research output: Contribution to journalArticlepeer-review

Abstract

Retinyl acetate alters glycoprotein synthesis in mouse epidermal cells in culture. Epidermal glycoproteins were enzymatically digested to glycopeptides and separated on DEAE Sephadex A50 columns using different concentrations of LiCl. There was a two-fold increase in incorporation of fucose and glucosamine in the 0.2 M LiCl fraction from cells treated for 3 weeks with 12.5 μg/ml retinyl acetate and 1.25% DMSO as compared with DMSO controls. No changes were noted in other fractions. The glycopeptide from A treated cells isolated on 0.2 M LiCl had a higher molecular weight than glycopeptides from that same fraction eluted by control cells. This isolated newly synthesized glycopeptide from vitamin A treated cells appears to be a single product by rechromatography on DEAE Sephadex A50 and Sephadex G100 columns.

Original languageEnglish (US)
Pages (from-to)106-110
Number of pages5
JournalExperimental cell research
Volume86
Issue number1
DOIs
StatePublished - May 1974
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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