Altered gene expression profiles in nasal respiratory epithelium reflect stable versus acute childhood asthma

Jesus R. Guajardo, Kathleen W. Schleifer, Michael O. Daines, Richard M. Ruddy, Bruce J. Aronow, Marsha Wills-Karp, Gurjit K Khurana Hershey

Research output: Contribution to journalArticle

Abstract

Background: Asthma is the most common chronic disease of childhood and has a strong genetic component. Objective: To identify gene expression signatures that reflect asthma-related processes and to determine whether these genes were similar or distinct between stable asthma and acute exacerbations in childhood, we profiled gene expression patterns in nasal respiratory epithelial cells. Methods: Children who had stable asthma (asthma-S; n = 10) and children experiencing an asthma exacerbation (asthma-E; n = 10) were recruited along with nonatopic children without asthma (n = 10). RNA was prepared from nasal respiratory epithelial cells isolated from each child, initially analyzed as pooled samples from the 3 groups, and further validated by using microarrays and RT-PCR with individual patient samples. Results: Distinct gene clusters were identifiable in individual and pooled asthma-S and asthma-E samples. Asthma-E samples demonstrated the strongest and most reproducible signatures, with 314 genes of 34,886 measured as present on the chip demonstrating induction or repression of greater than 2-fold with P

Original languageEnglish (US)
Pages (from-to)243-251
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume115
Issue number2
DOIs
Publication statusPublished - Feb 2005
Externally publishedYes

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Keywords

  • Asthma
  • Childhood
  • Genetics
  • Microarray

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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