Altered expression of mRNA profiles in blood of early-onset schizophrenia

Yong Xu, Yin Yao Shugart, Guoqiang Wang, Zaohuo Cheng, Chunhui Jin, Kai Zhang, Jun Wang, Hao Yu, Weihua Yue, Fuquan Zhang, Dai Zhang

Research output: Contribution to journalArticle

Abstract

To identify gene expression abnormalities in schizophrenia (SZ), we generated whole-genome gene expression profiles using microarrays on peripheral blood mononuclear cells (PBMCs) from 18 early-onset SZ cases and 12 controls. We detected 84 transcripts differentially expressed by diagnostic status, with 82 genes being upregulated and 2 downregulated. We identified two SZ associated gene coexpression modules (green and red), including 446 genes. The green module is positively correlated with SZ, encompassing predominantly up-regulated genes in SZ; while the red module was negatively correlated with disease status, involving mostly nominally down-regulated genes in SZ. The olfactory transduction pathway was the most enriched pathways for the genes within the two modules. The expression levels of several hub genes, including AKT1, BRCA1, CCDC134, UBD, and ZIC2 were validated using real-time quantitative PCR. Our findings indicate that mRNA coexpression abnormalities may serve as a promising mechanism underlying the development of SZ.

Original languageEnglish (US)
Article number16767
JournalScientific Reports
Volume6
DOIs
StatePublished - Jan 6 2016
Externally publishedYes

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Schizophrenia
Messenger RNA
Genes
Olfactory Pathways
Gene Regulatory Networks
Transcriptome
Real-Time Polymerase Chain Reaction
Blood Cells
Down-Regulation
Genome
Gene Expression

ASJC Scopus subject areas

  • General

Cite this

Xu, Y., Yao Shugart, Y., Wang, G., Cheng, Z., Jin, C., Zhang, K., ... Zhang, D. (2016). Altered expression of mRNA profiles in blood of early-onset schizophrenia. Scientific Reports, 6, [16767]. https://doi.org/10.1038/srep16767

Altered expression of mRNA profiles in blood of early-onset schizophrenia. / Xu, Yong; Yao Shugart, Yin; Wang, Guoqiang; Cheng, Zaohuo; Jin, Chunhui; Zhang, Kai; Wang, Jun; Yu, Hao; Yue, Weihua; Zhang, Fuquan; Zhang, Dai.

In: Scientific Reports, Vol. 6, 16767, 06.01.2016.

Research output: Contribution to journalArticle

Xu, Y, Yao Shugart, Y, Wang, G, Cheng, Z, Jin, C, Zhang, K, Wang, J, Yu, H, Yue, W, Zhang, F & Zhang, D 2016, 'Altered expression of mRNA profiles in blood of early-onset schizophrenia', Scientific Reports, vol. 6, 16767. https://doi.org/10.1038/srep16767
Xu Y, Yao Shugart Y, Wang G, Cheng Z, Jin C, Zhang K et al. Altered expression of mRNA profiles in blood of early-onset schizophrenia. Scientific Reports. 2016 Jan 6;6. 16767. https://doi.org/10.1038/srep16767
Xu, Yong ; Yao Shugart, Yin ; Wang, Guoqiang ; Cheng, Zaohuo ; Jin, Chunhui ; Zhang, Kai ; Wang, Jun ; Yu, Hao ; Yue, Weihua ; Zhang, Fuquan ; Zhang, Dai. / Altered expression of mRNA profiles in blood of early-onset schizophrenia. In: Scientific Reports. 2016 ; Vol. 6.
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