Altered expression of fibrosis genes in capsules of failed ahmed glaucoma valve implants

Alka Mahale, Maha W. Othman, Sami Al Shahwan, Ibrahim Al Jadaan, Ohood Owaydha, Zahid Khan, Deepak P. Edward

Research output: Contribution to journalArticle

Abstract

Purpose: Ahmed glaucoma valve (AGV) implant is an aqueous shunt device used to control intraocular pressure in glaucoma. Implant failure results from impervious encapsulation of the shunt plate causing increased hydraulic resistance and raised intraocular pressure. We hypothesized that deregulation of fibrosis pathway contributes to capsular resistance. We tested this by studying fibrosis related gene expression in failed AGV implants. Methods: Differential gene expression was examined in failed AGV capsules and compared to normal control tenon. Following total RNA extraction, 84 key genes in fibrosis pathway were examined by real-time PCR using RT2 Profiler PCR Array. Relative gene expression was calculated using ΔΔCt method. Gene specific TaqMan assays were used to validate select genes with ≥2 fold differential expression in the array expression profile. Results: We observed differential expression in several genes in the fibrosis pathway. Almost half (39/84) of examined genes showed ≥2 fold differential expression in majority of capsules examined on the array. TaqMan assays for select genes including CCN2 (CTGF), THBS1, SERPINE1, THBS2, COL3A1, MMP3, and IL1A in an increased validation sample set showed significant changes in expression (p value from

Original languageEnglish (US)
Article numbere0122409
JournalPLoS One
Volume10
Issue number4
DOIs
StatePublished - Apr 16 2015

Fingerprint

glaucoma
fibrosis
Glaucoma
Capsules
Fibrosis
Genes
Gene Expression
gene expression
Gene expression
genes
Intraocular Pressure
Assays
Glaucoma Drainage Implants
assays
encapsulation
Deregulation
Encapsulation
quantitative polymerase chain reaction
fluid mechanics
Real-Time Polymerase Chain Reaction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mahale, A., Othman, M. W., Al Shahwan, S., Al Jadaan, I., Owaydha, O., Khan, Z., & Edward, D. P. (2015). Altered expression of fibrosis genes in capsules of failed ahmed glaucoma valve implants. PLoS One, 10(4), [e0122409]. https://doi.org/10.1371/journal.pone.0122409

Altered expression of fibrosis genes in capsules of failed ahmed glaucoma valve implants. / Mahale, Alka; Othman, Maha W.; Al Shahwan, Sami; Al Jadaan, Ibrahim; Owaydha, Ohood; Khan, Zahid; Edward, Deepak P.

In: PLoS One, Vol. 10, No. 4, e0122409, 16.04.2015.

Research output: Contribution to journalArticle

Mahale, A, Othman, MW, Al Shahwan, S, Al Jadaan, I, Owaydha, O, Khan, Z & Edward, DP 2015, 'Altered expression of fibrosis genes in capsules of failed ahmed glaucoma valve implants', PLoS One, vol. 10, no. 4, e0122409. https://doi.org/10.1371/journal.pone.0122409
Mahale A, Othman MW, Al Shahwan S, Al Jadaan I, Owaydha O, Khan Z et al. Altered expression of fibrosis genes in capsules of failed ahmed glaucoma valve implants. PLoS One. 2015 Apr 16;10(4). e0122409. https://doi.org/10.1371/journal.pone.0122409
Mahale, Alka ; Othman, Maha W. ; Al Shahwan, Sami ; Al Jadaan, Ibrahim ; Owaydha, Ohood ; Khan, Zahid ; Edward, Deepak P. / Altered expression of fibrosis genes in capsules of failed ahmed glaucoma valve implants. In: PLoS One. 2015 ; Vol. 10, No. 4.
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