Altered cholesterol biosynthesis causes precocious neurogenesis in the developing mouse forebrain

Ashley M. Driver, Lisa E. Kratz, Richard I. Kelley, Rolf W. Stottmann

Research output: Contribution to journalArticlepeer-review

Abstract

We previously reported a mutation in the cholesterol biosynthesis gene, hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7rudolph), that results in striking embryonic forebrain dysgenesis. Here we describe abnormal patterns of neuroprogenitor proliferation in the mutant forebrain, namely, a decrease in mitotic cells within the ventricular zone (VZ) and an increase through the remainder of the cortex by E11.5. Further evidence suggests mutant cells undergo abnormal interkinetic nuclear migration (IKNM). Furthermore, intermediate progenitors are increased at the expense of apical progenitors by E12.5, and post-mitotic neurons are expanded by E14.5. In vitro primary neuron culture further supports our model of accelerated cortical differentiation in the mutant. Combined administration of a statin and dietary cholesterol in utero achieved partial reversal of multiple developmental abnormalities in the Hsd17b7rudolph embryo, including the forebrain. These results suggest that abnormally increased levels of specific cholesterol precursors in the Hsd17b7rudolph embryo cause cortical dysgenesis by altering patterns of neurogenesis.

Original languageEnglish (US)
Pages (from-to)69-82
Number of pages14
JournalNeurobiology of Disease
Volume91
DOIs
StatePublished - Jul 1 2016

Keywords

  • Cell fate
  • Cholesterol biosynthesis
  • Cortical neurogenesis
  • Development
  • Forebrain
  • Hsd17b7
  • Mouse
  • Neural progenitors
  • Neurogenesis
  • Statin

ASJC Scopus subject areas

  • Neurology

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