Altered chloride metabolism in cultured cystic fibrosis skin fibroblasts

P. M. Mattes, P. C. Maloney, J. W. Littlefield

Research output: Contribution to journalArticlepeer-review

Abstract

An abnormal regulation of chloride permeability has been described for epithelial cells from patients with cystic fibrosis (CF). To learn more about the biochemical basis of this inherited disease, we have studied chloride metabolism in cultured CF fibroblasts by comparing the efflux of 36Cl- from matched pairs of CF and normal fibroblasts. The rate constants describing 36Cl- efflux did not differ between the two cell types, but in each of the four pairs tested the amount of 36Cl- contained within CF cells was consistently reduced, by 25-30%, relative to normal cells. Comparisons of cell water content and 22Na+ efflux showed no differences between the two cell types, suggesting that overall intracellular chloride concentration is lower than normal in CF fibroblasts. Such data suggest that the CF gene defect is expressed in skin fibroblasts and that this defect may alter the regulation of intracellular Cl- concentration, perhaps through changes in Cl- permeability.

Original languageEnglish (US)
Pages (from-to)3009-3013
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number9
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • General

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