Altered amount and activity of superoxide dismutase in sickle cell anemia

L. Schacter, J. A. Warth, E. M. Gordon, A. Prasad, Bruce Klein

Research output: Contribution to journalArticle

Abstract

The amount and activity of superoxide dismutase (SOD) (EC 1.15.1.1) were measured in red cells collected from 50 white controls, 101 black controls, 50 patients with sickle hemoglobin (SS Hb), 12 with sickle trait, and 11 with other sickling hemoglobinopathies. Red cells from normal black subjects had more SOD amount and activity than normal whites (1.77 U/mg Hb and 2.96 μg/mg Hb vs. 1.47 U/mg Hb and 2.64 μg/mg Hb, respectively) or blacks with SS Hb or other sickling hemoglobinopathies. Patients with more severe manifestations of SS Hb had lower levels of SOD activity than those with milder symptoms but had the same amount of enzyme protein. Individuals with sickle trait had amounts and activities of SOD comparable to black controls. An alteration in defense to free radical oxygen may play a role in the severity of symptoms experienced by patients with homozygous siclke cell disease.

Original languageEnglish (US)
Pages (from-to)237-243
Number of pages7
JournalFASEB Journal
Volume2
Issue number3
StatePublished - 1988
Externally publishedYes

Fingerprint

Sickle Cell Anemia
Superoxide Dismutase
Sickle Hemoglobin
Hemoglobinopathies
Cells
Free Radicals
Oxygen
Enzymes
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Schacter, L., Warth, J. A., Gordon, E. M., Prasad, A., & Klein, B. (1988). Altered amount and activity of superoxide dismutase in sickle cell anemia. FASEB Journal, 2(3), 237-243.

Altered amount and activity of superoxide dismutase in sickle cell anemia. / Schacter, L.; Warth, J. A.; Gordon, E. M.; Prasad, A.; Klein, Bruce.

In: FASEB Journal, Vol. 2, No. 3, 1988, p. 237-243.

Research output: Contribution to journalArticle

Schacter, L, Warth, JA, Gordon, EM, Prasad, A & Klein, B 1988, 'Altered amount and activity of superoxide dismutase in sickle cell anemia', FASEB Journal, vol. 2, no. 3, pp. 237-243.
Schacter L, Warth JA, Gordon EM, Prasad A, Klein B. Altered amount and activity of superoxide dismutase in sickle cell anemia. FASEB Journal. 1988;2(3):237-243.
Schacter, L. ; Warth, J. A. ; Gordon, E. M. ; Prasad, A. ; Klein, Bruce. / Altered amount and activity of superoxide dismutase in sickle cell anemia. In: FASEB Journal. 1988 ; Vol. 2, No. 3. pp. 237-243.
@article{f64f595fbc484645896a5163632d75b1,
title = "Altered amount and activity of superoxide dismutase in sickle cell anemia",
abstract = "The amount and activity of superoxide dismutase (SOD) (EC 1.15.1.1) were measured in red cells collected from 50 white controls, 101 black controls, 50 patients with sickle hemoglobin (SS Hb), 12 with sickle trait, and 11 with other sickling hemoglobinopathies. Red cells from normal black subjects had more SOD amount and activity than normal whites (1.77 U/mg Hb and 2.96 μg/mg Hb vs. 1.47 U/mg Hb and 2.64 μg/mg Hb, respectively) or blacks with SS Hb or other sickling hemoglobinopathies. Patients with more severe manifestations of SS Hb had lower levels of SOD activity than those with milder symptoms but had the same amount of enzyme protein. Individuals with sickle trait had amounts and activities of SOD comparable to black controls. An alteration in defense to free radical oxygen may play a role in the severity of symptoms experienced by patients with homozygous siclke cell disease.",
author = "L. Schacter and Warth, {J. A.} and Gordon, {E. M.} and A. Prasad and Bruce Klein",
year = "1988",
language = "English (US)",
volume = "2",
pages = "237--243",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Altered amount and activity of superoxide dismutase in sickle cell anemia

AU - Schacter, L.

AU - Warth, J. A.

AU - Gordon, E. M.

AU - Prasad, A.

AU - Klein, Bruce

PY - 1988

Y1 - 1988

N2 - The amount and activity of superoxide dismutase (SOD) (EC 1.15.1.1) were measured in red cells collected from 50 white controls, 101 black controls, 50 patients with sickle hemoglobin (SS Hb), 12 with sickle trait, and 11 with other sickling hemoglobinopathies. Red cells from normal black subjects had more SOD amount and activity than normal whites (1.77 U/mg Hb and 2.96 μg/mg Hb vs. 1.47 U/mg Hb and 2.64 μg/mg Hb, respectively) or blacks with SS Hb or other sickling hemoglobinopathies. Patients with more severe manifestations of SS Hb had lower levels of SOD activity than those with milder symptoms but had the same amount of enzyme protein. Individuals with sickle trait had amounts and activities of SOD comparable to black controls. An alteration in defense to free radical oxygen may play a role in the severity of symptoms experienced by patients with homozygous siclke cell disease.

AB - The amount and activity of superoxide dismutase (SOD) (EC 1.15.1.1) were measured in red cells collected from 50 white controls, 101 black controls, 50 patients with sickle hemoglobin (SS Hb), 12 with sickle trait, and 11 with other sickling hemoglobinopathies. Red cells from normal black subjects had more SOD amount and activity than normal whites (1.77 U/mg Hb and 2.96 μg/mg Hb vs. 1.47 U/mg Hb and 2.64 μg/mg Hb, respectively) or blacks with SS Hb or other sickling hemoglobinopathies. Patients with more severe manifestations of SS Hb had lower levels of SOD activity than those with milder symptoms but had the same amount of enzyme protein. Individuals with sickle trait had amounts and activities of SOD comparable to black controls. An alteration in defense to free radical oxygen may play a role in the severity of symptoms experienced by patients with homozygous siclke cell disease.

UR - http://www.scopus.com/inward/record.url?scp=0023833103&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023833103&partnerID=8YFLogxK

M3 - Article

C2 - 3350236

AN - SCOPUS:0023833103

VL - 2

SP - 237

EP - 243

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -