Alterations of yeast artificial chromosome transgenic sequences in stretched embryonic stem-cell chromatin visualized by fluorescence in situ hybridization

C. Rosenberg; A. K. Voltz; B. T. Lamb; G. Stetten; J. D. Gearhart

doi:10.1159/000134459

Alterations of yeast artificial chromosome transgenic sequences in stretched embryonic stem-cell chromatin visualized by fluorescence in situ hybridization

C. Rosenberg, A. K. Voltz, B. T. Lamb, G. Stetten, J. D. Gearhart

School of Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Transgenic mice have been generated from embryonic stem (ES) cells carrying functional genes cloned within yeast artificial chromosomes (YACs). Information on the integrity and organization of the inserted sequences, including the, number of copies and their orientation to each other, is still limited by current methods. We have applied fluorescence in situ hybridization to stretched chromatin preparations from YAC-transfected ES cells to analyze the organization and copy number of the integrated sequences.

Original language	English (US)
Pages (from-to)	67-70
Number of pages	4
Journal	Cytogenetics and cell genetics
Volume	75
Issue number	1
DOIs	https://doi.org/10.1159/000134459
State	Published - Jan 1 1996

ASJC Scopus subject areas

Genetics
Cell Biology

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10.1159/000134459

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abstract = "Transgenic mice have been generated from embryonic stem (ES) cells carrying functional genes cloned within yeast artificial chromosomes (YACs). Information on the integrity and organization of the inserted sequences, including the, number of copies and their orientation to each other, is still limited by current methods. We have applied fluorescence in situ hybridization to stretched chromatin preparations from YAC-transfected ES cells to analyze the organization and copy number of the integrated sequences.",

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AU - Stetten, G.

AU - Gearhart, J. D.

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AB - Transgenic mice have been generated from embryonic stem (ES) cells carrying functional genes cloned within yeast artificial chromosomes (YACs). Information on the integrity and organization of the inserted sequences, including the, number of copies and their orientation to each other, is still limited by current methods. We have applied fluorescence in situ hybridization to stretched chromatin preparations from YAC-transfected ES cells to analyze the organization and copy number of the integrated sequences.

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Alterations of yeast artificial chromosome transgenic sequences in stretched embryonic stem-cell chromatin visualized by fluorescence in situ hybridization

Abstract

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