Alterations of neutral glycolipids in cells infected with syncytium producing mutants of herpes simplex virus type 1

M. A. Ruhlig, S. Person

Research output: Contribution to journalArticle

Abstract

Seven syncytium producing mutants of herpes simplex virus type 1 (KOS strain), plus two syncytial strains obtained elsewhere, were used to compare the incorporation of labeled galactose into neutral glycolipids of mock-infected, wild-type-infected, and syncytially infected human embryonic lung cells. Five predominant cellular glycolipid species were observed, denoted GL-1 through GL-5 in order of increasing oligosaccharide chain length; for example, GL-1 and GL-2 correspond to glycolipids that contain mono- and disaccharide units, respectively. Wild-type virus infection caused an increase in galactose incorporation into GL-1 and GL-2 relative to GL-3 through GL-5. For a single labeling interval from 4 to 10 h after adsorption, syncytial infections generally resulted in a relatively greater incorporation into more complex glycolipids than did wild-type infections. One mutant, syn 20, was compared with wild-type virus throughout infection by using a series of shorter labeling pulses and appeared to delay by at least 2 h the alterations observed during wild-type infections. These alterations are apparently due to defects in synthesis, since prelabeled cellular glycolipids were not differentially degraded during mock or virus infection.

Original languageEnglish (US)
Pages (from-to)602-608
Number of pages7
JournalJournal of Virology
Volume24
Issue number2
Publication statusPublished - 1977
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

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