Alterations in the superoxide dismutase gene of an isoniazid-resistant strain of Mycobacterium tuberculosis

Y. Zhang, M. J. Garcia, R. Lathigra, B. Allen, C. Moreno, J. D.A. Van Embden, D. Young

Research output: Contribution to journalArticle

Abstract

Genetic analysis of a set of six Mycobacterium tuberculosis strains differing in virulence for the guinea pig revealed an altered restriction enzyme fragmentation pattern associated with the superoxide dismutase (SOD) gene in a low-virulence, isoniazid-resistant strain. In addition, it was found that the SOD enzyme produced by the isoniazid-resistant strain differed in its electrophoretic mobility from the SOD of other M. tuberculosis strains. Detailed analysis of these strain-specific differences showed that the restriction fragment length polymorphism resulted from the presence of a copy of a repetitive element 552 bp upstream of the SOD gene and that the anomalous electrophoretic mobility arose from a single nucleotide change, resulting in replacement of an aspartic acid residue by histidine in the SOD enzyme of the isoniazid-resistant strain. Possible relationships between genetic changes and strain-dependent differences in virulence are discussed.

Original languageEnglish (US)
Pages (from-to)2160-2165
Number of pages6
JournalInfection and immunity
Volume60
Issue number6
StatePublished - 1992

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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    Zhang, Y., Garcia, M. J., Lathigra, R., Allen, B., Moreno, C., Van Embden, J. D. A., & Young, D. (1992). Alterations in the superoxide dismutase gene of an isoniazid-resistant strain of Mycobacterium tuberculosis. Infection and immunity, 60(6), 2160-2165.