Alterations in soluble and leukocyte surface L-selectin (CD 62L) in hemodialysis patients

Hamid Rabb, S. J. Agosti, P. A. Bittle, M. Fernandez, G. Ramirez, T. F. Tedder

Research output: Contribution to journalArticle

Abstract

Hemodialysis (HD) patients can develop acute reactions during treatment as well as increased long-term susceptibility to infections and malignancy. Leukocyte-membrane interactions may contribute to these processes. The effects of a single HD session on L-selectin, a leukocyte adhesion molecule for endothelium, were examined. Serum levels of soluble L-selectin were measured in 23 patients by enzyme-linked immunosorbent assay before and after a 3-h dialysis session. There was a statistically significant increase in soluble L-selectin from 1.36 ± 0.12 (SE) to 1.57 ± 0.18 μg/mL (P <0.01). An increase in shed L-selectin was observed for the 'venous' compared with the 'arterial' port of the dialyser (P <0.01) 15 min into HD. Soluble L-selectin levels were found to remain increased at 3 h after treatment. Leukocyte-bound L-selectin and CD11b was examined by the use of flow cytometry. Neutrophil L-selectin decreased to 69 ± 7% at 15 min (P <0.01) and then rebounded to 98 ± 7% at 180 min. Monocyte and lymphocyte L-selectin did not decrease. Because L-selectin is important for leukocyte attachment to endothelium at sites of inflammation, alterations of shed L-selectin and cell-surface L-selectin levels may play a role in the immunologic consequences of HD treatment.

Original languageEnglish (US)
Pages (from-to)1445-1450
Number of pages6
JournalJournal of the American Society of Nephrology
Volume6
Issue number5
Publication statusPublished - 1995
Externally publishedYes

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Keywords

  • Adhesion
  • Immunodeficiency
  • Membrane
  • Receptors
  • Uremia

ASJC Scopus subject areas

  • Nephrology

Cite this

Rabb, H., Agosti, S. J., Bittle, P. A., Fernandez, M., Ramirez, G., & Tedder, T. F. (1995). Alterations in soluble and leukocyte surface L-selectin (CD 62L) in hemodialysis patients. Journal of the American Society of Nephrology, 6(5), 1445-1450.