Alterations in nuclear structure and expression of proPSA predict differences between native Japanese and Japanese-American prostate cancer

Robert W. Veltri, Masood A. Khan, Cameron Marlow, M. Craig Miller, Stephen D. Mikolajczyk, Munekado Kojima, Alan Wayne Partin, Leonard S. Marks

Research output: Contribution to journalArticle

Abstract

Objectives: To differentiate the benign and/or malignant epithelial cells in prostate cancer (PCa) glands of native Japanese (NJ) and Japanese-American (JA) men using biomarkers. Methods: Tissue microarrays from radical prostatectomy specimens of cancerous and adjacent benign areas from 25 NJ and 25 JA prostate glands were studied. Image analysis was used to quantify total prostate-specific antigen (PSA) and proPSA immunohistochemical staining, as well as the variance of several morphometric features from Feulgen-stained epithelial cell nuclei. Logistic regression analysis was applied to determine whether quantitative nuclear grade (QNG) calculations and PSA immunohistochemical staining could differentiate the two test groups. Results: The QNG model differentiated changes in the benign epithelium of the two Japanese groups with an area under the receiver operating characteristic curve of 84% and accuracy of 82% (P = 0.0001). A second QNG model differentiated changes in the malignant epithelium of the two groups with an area under the receiver operating characteristic curve of 84% and accuracy of 76% (P = 0.0023). Logistic regression models combining proPSA immunohistochemical data and QNG from either benign or malignant tissue components yielded areas under the receiver operating characteristic curve of 96% and 91% (P

Original languageEnglish (US)
Pages (from-to)898-904
Number of pages7
JournalUrology
Volume68
Issue number4
DOIs
StatePublished - Oct 2006

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Asian Americans
ROC Curve
Prostatic Neoplasms
Logistic Models
Prostate-Specific Antigen
Epithelium
Epithelial Cells
Staining and Labeling
Prostatectomy
Cell Nucleus
Prostate
Biomarkers
Regression Analysis

ASJC Scopus subject areas

  • Urology

Cite this

Veltri, R. W., Khan, M. A., Marlow, C., Miller, M. C., Mikolajczyk, S. D., Kojima, M., ... Marks, L. S. (2006). Alterations in nuclear structure and expression of proPSA predict differences between native Japanese and Japanese-American prostate cancer. Urology, 68(4), 898-904. https://doi.org/10.1016/j.urology.2006.05.008

Alterations in nuclear structure and expression of proPSA predict differences between native Japanese and Japanese-American prostate cancer. / Veltri, Robert W.; Khan, Masood A.; Marlow, Cameron; Miller, M. Craig; Mikolajczyk, Stephen D.; Kojima, Munekado; Partin, Alan Wayne; Marks, Leonard S.

In: Urology, Vol. 68, No. 4, 10.2006, p. 898-904.

Research output: Contribution to journalArticle

Veltri, Robert W. ; Khan, Masood A. ; Marlow, Cameron ; Miller, M. Craig ; Mikolajczyk, Stephen D. ; Kojima, Munekado ; Partin, Alan Wayne ; Marks, Leonard S. / Alterations in nuclear structure and expression of proPSA predict differences between native Japanese and Japanese-American prostate cancer. In: Urology. 2006 ; Vol. 68, No. 4. pp. 898-904.
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abstract = "Objectives: To differentiate the benign and/or malignant epithelial cells in prostate cancer (PCa) glands of native Japanese (NJ) and Japanese-American (JA) men using biomarkers. Methods: Tissue microarrays from radical prostatectomy specimens of cancerous and adjacent benign areas from 25 NJ and 25 JA prostate glands were studied. Image analysis was used to quantify total prostate-specific antigen (PSA) and proPSA immunohistochemical staining, as well as the variance of several morphometric features from Feulgen-stained epithelial cell nuclei. Logistic regression analysis was applied to determine whether quantitative nuclear grade (QNG) calculations and PSA immunohistochemical staining could differentiate the two test groups. Results: The QNG model differentiated changes in the benign epithelium of the two Japanese groups with an area under the receiver operating characteristic curve of 84{\%} and accuracy of 82{\%} (P = 0.0001). A second QNG model differentiated changes in the malignant epithelium of the two groups with an area under the receiver operating characteristic curve of 84{\%} and accuracy of 76{\%} (P = 0.0023). Logistic regression models combining proPSA immunohistochemical data and QNG from either benign or malignant tissue components yielded areas under the receiver operating characteristic curve of 96{\%} and 91{\%} (P",
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AU - Veltri, Robert W.

AU - Khan, Masood A.

AU - Marlow, Cameron

AU - Miller, M. Craig

AU - Mikolajczyk, Stephen D.

AU - Kojima, Munekado

AU - Partin, Alan Wayne

AU - Marks, Leonard S.

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N2 - Objectives: To differentiate the benign and/or malignant epithelial cells in prostate cancer (PCa) glands of native Japanese (NJ) and Japanese-American (JA) men using biomarkers. Methods: Tissue microarrays from radical prostatectomy specimens of cancerous and adjacent benign areas from 25 NJ and 25 JA prostate glands were studied. Image analysis was used to quantify total prostate-specific antigen (PSA) and proPSA immunohistochemical staining, as well as the variance of several morphometric features from Feulgen-stained epithelial cell nuclei. Logistic regression analysis was applied to determine whether quantitative nuclear grade (QNG) calculations and PSA immunohistochemical staining could differentiate the two test groups. Results: The QNG model differentiated changes in the benign epithelium of the two Japanese groups with an area under the receiver operating characteristic curve of 84% and accuracy of 82% (P = 0.0001). A second QNG model differentiated changes in the malignant epithelium of the two groups with an area under the receiver operating characteristic curve of 84% and accuracy of 76% (P = 0.0023). Logistic regression models combining proPSA immunohistochemical data and QNG from either benign or malignant tissue components yielded areas under the receiver operating characteristic curve of 96% and 91% (P

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