Alterations in cellular gene expression without changes in nuclear matrix protein content

Jill Macoska, Carol N. Hoover, Kenneth J. Pienta

Research output: Contribution to journalArticle

Abstract

Cell metabolism and function are modulated in part by cell and nuclear shape. Nuclear shape is controlled by the nuclear matrix, the RNA‐protein skeleton the nucleus, and its interactions with cytoskeletal systems such as intermediate filaments and actin microfilaments. The nuclear matrix plays an important role in cell function and gene expression because active genes are bound to the nuclear matrix whereas inactive genes are not. It is unknown, however, how genes move on and off the matrix, and whether these events require compositional protein changes, i.e., alterations in protein content of the nuclear matrix, or other, more subtle alterations and/or modificatins. The purpose of this investigation was to begin to determine how nuclear matrix protein composition is related to gene expression. We demonstrate that gene expression can change without apparent changes in the protein composition of the nuclear matrix in MCF10A breast epithelial cells.

Original languageEnglish (US)
Pages (from-to)502-509
Number of pages8
JournalJournal of cellular biochemistry
Volume56
Issue number4
DOIs
StatePublished - Dec 1994
Externally publishedYes

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Keywords

  • MCF10A
  • actin
  • gene expression
  • nuclear matrix
  • ras

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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