Alteration of the blood-retinal barrier by sodium iodate: Kinetic vitreous fluorophotometry and horseradish peroxidase trace studies

Brad Anstadt, Norman P. Blair, Mark Rusin, Jose G. Cunha-Vaz, Mark O.M. Tso

Research output: Contribution to journalArticle

Abstract

The intraocular dynamics of fluorescein were examined in the rat eye after systemic administration of graded concentrations of sodium iodate. A comparatively selective alteration of the outward transport component was achieved immediately after systemic administration of sodium iodate (20 mg/kg), as demonstrated by a marked reduction of the rate of fluorescein loss from the vitreous (normal, t21=1·4 hr; n=9; sodium iodate, t21=4·8 hr; n=6). A different type of barrier alteration was observed when higher doses of sodium iodate wereused (60 mg/kg). The penetration of fluorescein into the vitreous increased dramatically, approaching plasma levels and following them thereafter indicating that a situation of free diffusion was achieved. The structural changes, examined by electron microscopy using horseradish peroxidase,correlated well with the intraocular fluorescein dynamics. There was moderate damage in the retinal pigment epithelium immediately after the lower dose of sodium iodate was injected and complete breakdown of the retinal pigment epithelium, with extensive cellular destruction and direct apposition between photoreceptors and Bruch's membrane, when higher doses of sodium iodate were administered.

Original languageEnglish (US)
Pages (from-to)653-662
Number of pages10
JournalExperimental eye research
Volume35
Issue number6
DOIs
StatePublished - Dec 1982
Externally publishedYes

Keywords

  • Blood-retinal barrier
  • horseradish peroxidase
  • sodium iodate
  • vitreous fluorophotometry

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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