Alteration of human neuroblastoma cell morphology and neurite extension with micropatterns

In Hong Yang, Carlos C. Co, Chia Chi Ho

Research output: Contribution to journalArticle

Abstract

The spatial orientation of nerve cells plays a pivotal role in nerve regeneration. Here we report a new method for regulating neuronal cell morphology and guiding neurite extension on standard tissue culture dishes. Random copolymers of oligoethyleneglycol methacrylate and methacrylic acid [poly(OEGMA-co-MA)], microcontact printed on standard tissue culture dishes, resist cell attachment and remain intact in serum-containing medium for up to 2 weeks. Cell viability assay of SH-SY5Y cells demonstrated that poly(OEGMA-co-MA) on the substrate or in solution has no cytotoxic effect. When retinoic acid was added to SH-SY5Y cells, they extended neurites along the line patterns that are significantly longer than cells cultured on non-patterned culture dishes. The ability to guide neurite extension with micrometer precision is valuable for guiding directional growth of neurites and path finding of regenerating nerves.

Original languageEnglish (US)
Pages (from-to)6599-6609
Number of pages11
JournalBiomaterials
Volume26
Issue number33
DOIs
StatePublished - Nov 2005
Externally publishedYes

Fingerprint

Tissue culture
Neurites
Neuroblastoma
Methacrylates
Acids
Tretinoin
Neurons
Assays
Copolymers
Cells
Nerve Regeneration
Substrates
Cultured Cells
Cell Survival
poly(poly(ethylene glycol)methacrylate)
Growth
Serum
methacrylic acid

Keywords

  • Microcontact printing
  • Microfabrication
  • Neurite extension
  • Neuron
  • SH-SY5Y

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Alteration of human neuroblastoma cell morphology and neurite extension with micropatterns. / Yang, In Hong; Co, Carlos C.; Ho, Chia Chi.

In: Biomaterials, Vol. 26, No. 33, 11.2005, p. 6599-6609.

Research output: Contribution to journalArticle

Yang, In Hong ; Co, Carlos C. ; Ho, Chia Chi. / Alteration of human neuroblastoma cell morphology and neurite extension with micropatterns. In: Biomaterials. 2005 ; Vol. 26, No. 33. pp. 6599-6609.
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