Alphoid DNA polymorphisms for chromosome 21 can be distinguished from those of chromosome 13 using probes homologous to both

Ethylin Wang Jabs; Andrew C. Warren; Eugene W. Taylor; Claudia R. Colyer; Deborah A. Meyers; Stylianos E. Antonarakis

doi:10.1016/0888-7543(91)90231-3

Alphoid DNA polymorphisms for chromosome 21 can be distinguished from those of chromosome 13 using probes homologous to both

Ethylin Wang Jabs, Andrew C. Warren, Eugene W. Taylor, Claudia R. Colyer, Deborah A. Meyers, Stylianos E. Antonarakis

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Although alphoid DNA sequences shared among acrocentric chromosomes have been identified, no human chromosome 21-specific sequence has been isolated from the centromeric region. To identify alphoid DNA restriction fragment length polymorphisms (RFLPs) specific for chromosome 21, we hybridized human genomic DNA with alphoid DNA probes [L1.26; aRI(680),21-208] shared by chromosomes 13 and 21. We detected RFLPs with restriction enzymes EcoRI, HaeIII, MboI, StuI, and TaqI. The segregation of these RFLPs was analyzed in the 40 CEPH families. Linkage analysis between these RFLPs and loci previously mapped to either chromosome 13 or 21 revealed RFLPs that appear to be specific to chromosome 21. These polymorphisms may be useful as genetic markers of the centromeric region of chromosome 21. Different alphoid loci within the centromeric region of chromosome 13 were identified.

Original language	English (US)
Pages (from-to)	141-146
Number of pages	6
Journal	Genomics
Volume	9
Issue number	1
DOIs	https://doi.org/10.1016/0888-7543(91)90231-3
State	Published - Jan 1991
Externally published	Yes

ASJC Scopus subject areas

Genetics

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@article{13919dad253c42b0a43adb4db7c6d895,

title = "Alphoid DNA polymorphisms for chromosome 21 can be distinguished from those of chromosome 13 using probes homologous to both",

abstract = "Although alphoid DNA sequences shared among acrocentric chromosomes have been identified, no human chromosome 21-specific sequence has been isolated from the centromeric region. To identify alphoid DNA restriction fragment length polymorphisms (RFLPs) specific for chromosome 21, we hybridized human genomic DNA with alphoid DNA probes [L1.26; aRI(680),21-208] shared by chromosomes 13 and 21. We detected RFLPs with restriction enzymes EcoRI, HaeIII, MboI, StuI, and TaqI. The segregation of these RFLPs was analyzed in the 40 CEPH families. Linkage analysis between these RFLPs and loci previously mapped to either chromosome 13 or 21 revealed RFLPs that appear to be specific to chromosome 21. These polymorphisms may be useful as genetic markers of the centromeric region of chromosome 21. Different alphoid loci within the centromeric region of chromosome 13 were identified.",

author = "Jabs, {Ethylin Wang} and Warren, {Andrew C.} and Taylor, {Eugene W.} and Colyer, {Claudia R.} and Meyers, {Deborah A.} and Antonarakis, {Stylianos E.}",

note = "Funding Information: We areg ratefult o Dr. Howcock and Dr. Farrer for allowing us to use their chromosome 13 data from the C.EPH dat.ahase. This study was supported by NIH Grants HD-22819 (F:.W.,J., E.W.T.), GM-41015. HD-24606. NS-26454, and GM-2842X. A.C.W. was supported by a Clinician Scientist Award from the W. M. Keck Foundation, and is gratetill to the Alzheimer{\textquoteright}s Association for interest and support.",

year = "1991",

month = jan,

doi = "10.1016/0888-7543(91)90231-3",

language = "English (US)",

volume = "9",

pages = "141--146",

journal = "Genomics",

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T1 - Alphoid DNA polymorphisms for chromosome 21 can be distinguished from those of chromosome 13 using probes homologous to both

AU - Jabs, Ethylin Wang

AU - Warren, Andrew C.

AU - Taylor, Eugene W.

AU - Colyer, Claudia R.

AU - Meyers, Deborah A.

AU - Antonarakis, Stylianos E.

N1 - Funding Information: We areg ratefult o Dr. Howcock and Dr. Farrer for allowing us to use their chromosome 13 data from the C.EPH dat.ahase. This study was supported by NIH Grants HD-22819 (F:.W.,J., E.W.T.), GM-41015. HD-24606. NS-26454, and GM-2842X. A.C.W. was supported by a Clinician Scientist Award from the W. M. Keck Foundation, and is gratetill to the Alzheimer’s Association for interest and support.

PY - 1991/1

Y1 - 1991/1

N2 - Although alphoid DNA sequences shared among acrocentric chromosomes have been identified, no human chromosome 21-specific sequence has been isolated from the centromeric region. To identify alphoid DNA restriction fragment length polymorphisms (RFLPs) specific for chromosome 21, we hybridized human genomic DNA with alphoid DNA probes [L1.26; aRI(680),21-208] shared by chromosomes 13 and 21. We detected RFLPs with restriction enzymes EcoRI, HaeIII, MboI, StuI, and TaqI. The segregation of these RFLPs was analyzed in the 40 CEPH families. Linkage analysis between these RFLPs and loci previously mapped to either chromosome 13 or 21 revealed RFLPs that appear to be specific to chromosome 21. These polymorphisms may be useful as genetic markers of the centromeric region of chromosome 21. Different alphoid loci within the centromeric region of chromosome 13 were identified.

AB - Although alphoid DNA sequences shared among acrocentric chromosomes have been identified, no human chromosome 21-specific sequence has been isolated from the centromeric region. To identify alphoid DNA restriction fragment length polymorphisms (RFLPs) specific for chromosome 21, we hybridized human genomic DNA with alphoid DNA probes [L1.26; aRI(680),21-208] shared by chromosomes 13 and 21. We detected RFLPs with restriction enzymes EcoRI, HaeIII, MboI, StuI, and TaqI. The segregation of these RFLPs was analyzed in the 40 CEPH families. Linkage analysis between these RFLPs and loci previously mapped to either chromosome 13 or 21 revealed RFLPs that appear to be specific to chromosome 21. These polymorphisms may be useful as genetic markers of the centromeric region of chromosome 21. Different alphoid loci within the centromeric region of chromosome 13 were identified.

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Alphoid DNA polymorphisms for chromosome 21 can be distinguished from those of chromosome 13 using probes homologous to both

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