TY - JOUR
T1 - Alpha-V/Beta-3 and Alpha-V/Beta-5 Integrin distribution in neoplastic kidney
AU - Rabb, Hamid
AU - Barroso-Vicens, Elvira
AU - Adams, Richard
AU - Pow-Sang, Julio
AU - Ramirez, German
PY - 1996/1/1
Y1 - 1996/1/1
N2 - Integrins are transmembrane glycoproteins that mediate cell-cell and cell-matrix interactions. Altered integrin expression may contribute to tumor progression, invasiveness and metastases. The alpha-V/beta-3 (α>vβ>3; osteopontin/ vitronectin receptor) has recently been implicated in neovascularization and tumor-induced angiogenesis. α>v-Subunit also associates with β>5 to form an α>vβ>5-complex, another vitronectin receptor. We studied tissue distribution of α>v β>3 and α>v β>5-integrins, as well as α>1- and β>1-subunits in nephrectomy samples from 7 subjects with localized renal cell carcinoma. Grossly and histologically uninvolved regions (‘normal’) from the same nephrectomy specimens were used for comparison. Integrin expression was studied with specific monoclonal antibodies and the immunoperoxidase technique. α>vβ>3 was expressed in the glomerular epithelial cells, Bowman’s capsule, vascular endothelium, and weakly in tubular epithelial cells. α>vβ>5 had a similar distribution except for minimal expression on vascular endothelium. α>1-Expression was observed in mesangium and but weakly in Bowman’s capsule. β>1-Expression was seen in glomerular epithelial cells, Bowman’s capsule, vascular epithelium and tubular epithelial cells. Unlike in ‘normals’, neoplastic expression was more heterogeneous. α>vβ>3 was expressed in tumor cells in 4/7 cases, vascular endothelium in 6/6, and in stroma in 4/7. α>vβ>5 was weakly expressed in tumor cells in 4/5, vascular endothelium in 5/5, and stroma in 4/5 cases. α>1-Expression was seen in tumor cells in 3/7, vascular endothelium in 4/7 and in stroma in 7/7 cases. β>1-Expression was seen in tumor cells in 7/7 cases, vascular endothelium in 7/7, and in stroma in 4/7 cases. This study delineates the pattern of expression of the α>vβ>3- and α>vβ>5-integrins in ‘normal’ and neoplastic human kidney. Variations in α>vβ>3- and α>vβ>5-integrin expression may play a role in normal and neoplastic processes of the kidney.
AB - Integrins are transmembrane glycoproteins that mediate cell-cell and cell-matrix interactions. Altered integrin expression may contribute to tumor progression, invasiveness and metastases. The alpha-V/beta-3 (α>vβ>3; osteopontin/ vitronectin receptor) has recently been implicated in neovascularization and tumor-induced angiogenesis. α>v-Subunit also associates with β>5 to form an α>vβ>5-complex, another vitronectin receptor. We studied tissue distribution of α>v β>3 and α>v β>5-integrins, as well as α>1- and β>1-subunits in nephrectomy samples from 7 subjects with localized renal cell carcinoma. Grossly and histologically uninvolved regions (‘normal’) from the same nephrectomy specimens were used for comparison. Integrin expression was studied with specific monoclonal antibodies and the immunoperoxidase technique. α>vβ>3 was expressed in the glomerular epithelial cells, Bowman’s capsule, vascular endothelium, and weakly in tubular epithelial cells. α>vβ>5 had a similar distribution except for minimal expression on vascular endothelium. α>1-Expression was observed in mesangium and but weakly in Bowman’s capsule. β>1-Expression was seen in glomerular epithelial cells, Bowman’s capsule, vascular epithelium and tubular epithelial cells. Unlike in ‘normals’, neoplastic expression was more heterogeneous. α>vβ>3 was expressed in tumor cells in 4/7 cases, vascular endothelium in 6/6, and in stroma in 4/7. α>vβ>5 was weakly expressed in tumor cells in 4/5, vascular endothelium in 5/5, and stroma in 4/5 cases. α>1-Expression was seen in tumor cells in 3/7, vascular endothelium in 4/7 and in stroma in 7/7 cases. β>1-Expression was seen in tumor cells in 7/7 cases, vascular endothelium in 7/7, and in stroma in 4/7 cases. This study delineates the pattern of expression of the α>vβ>3- and α>vβ>5-integrins in ‘normal’ and neoplastic human kidney. Variations in α>vβ>3- and α>vβ>5-integrin expression may play a role in normal and neoplastic processes of the kidney.
KW - Cancer
KW - Integrins
KW - Kidney
KW - Osteopontin
KW - Tubules
KW - Vitronectin
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U2 - 10.1159/000169032
DO - 10.1159/000169032
M3 - Article
C2 - 8886177
AN - SCOPUS:0029846918
SN - 0250-8095
VL - 16
SP - 402
EP - 408
JO - American Journal of Nephrology
JF - American Journal of Nephrology
IS - 5
ER -