Allospecific dr gene expression on the human thyroid cell

S. M. Dower, L. A. Piccinini, S. H. Roman, R. Safirstein, T. F. Davies

Research output: Contribution to journalArticlepeer-review

Abstract

Human thyroid epithelial cells, stimulated with recombinant gamma interferon (IF) (100U/ml), were HLA-DR typed by means of antibody-dependent, complement-mediated cytotoxicity. The thyroid cell lysis reaction patterns were found to be similar to those obtained with autologous peripheral B cells in four of five separate experiments. Since the cytotoxic reactions with thyroid cells were sometimes incomplete with many antisera, a two-color fluorescence technique was developed to measure the specificity of the antibody-dependent, complement-mediated lysis. Using constitutively HLA-D antigen-positive Raji B lymphoblastoid cells as controls, we showed that non-polymorphic HLA-D and HLA-DR determinants could be detected on the thyroid cell surfaces by this cell lysis approach. In addition, gamma IF stimulated thyroid cells from a DR3 positive individual were lysed to a significantly greater degree than unstimulated autologous thyroid cells with the use of a specific monoclonal antibody to HLA-DR3, thus confirming the original HLA typing studies. These data demonstrate, for the first time, that the expression of HLA-DR antigen on the surface of human thyroid cells is allospecific and may, therefore, play an important role in the immunopathology of autoimmune thyroid disease.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalAutoimmunity
Volume1
Issue number1
DOIs
StatePublished - 1988
Externally publishedYes

Keywords

  • Autoimmunity
  • HLA
  • Immunopathology
  • Thyroid

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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