Alloreactive CD8+ T Cells Can Recognize Unusual, Rare, and Unique Processed Peptide/MHC Complexes

Subramaniam Malarkannan, Federico Gonzalez, Vu Nguyen, Gerald Adair, Nilabh Shastri

Research output: Contribution to journalArticle

Abstract

The identity and abundance of self-peptide/MHC class I complexes that serve as ligands for alloreactive T cells remain largely unknown. Using the Kb-restricted, alloreactive T cells as a probe, the Ag precursor gene, adenosine phosphoribosyl transferase (APRT), was isolated by expression cloning. Its naturally processed product was identified as the SLVELTSL (SEL8) octapeptide. The SEL8 peptide shared five residues with the previously identified SVVEFSSL (JAL8) peptide that stimulated the same T cell, but lacked the critical phenylalanine/tyrosine residue at the primary p5 anchor position. Despite the absence of this key conserved anchor residue, SEL8 was bound tightly by Kb MHC and yet was expressed at less than 10 copies/cell. Mutations in the donor APRT gene in the APC caused a concomitant loss in the ability of APCs to stimulate T cells. The results confirm that the display of peptide/MHC complexes in cells exceeds the predictions based upon consensus motifs, and that CD8+ alloreactive and conventional Ag-specific T cells are indistinguishable in their ability to recognize unique and rare peptide/MHC class I complexes.

Original languageEnglish (US)
Pages (from-to)4464-4473
Number of pages10
JournalJournal of Immunology
Volume157
Issue number10
StatePublished - Nov 15 1996
Externally publishedYes

Fingerprint

T-Lymphocytes
Peptides
Transferases
Adenosine
APC Genes
Phenylalanine
Tyrosine
Organism Cloning
Ligands
Mutation
Genes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Alloreactive CD8+ T Cells Can Recognize Unusual, Rare, and Unique Processed Peptide/MHC Complexes. / Malarkannan, Subramaniam; Gonzalez, Federico; Nguyen, Vu; Adair, Gerald; Shastri, Nilabh.

In: Journal of Immunology, Vol. 157, No. 10, 15.11.1996, p. 4464-4473.

Research output: Contribution to journalArticle

Malarkannan, S, Gonzalez, F, Nguyen, V, Adair, G & Shastri, N 1996, 'Alloreactive CD8+ T Cells Can Recognize Unusual, Rare, and Unique Processed Peptide/MHC Complexes', Journal of Immunology, vol. 157, no. 10, pp. 4464-4473.
Malarkannan, Subramaniam ; Gonzalez, Federico ; Nguyen, Vu ; Adair, Gerald ; Shastri, Nilabh. / Alloreactive CD8+ T Cells Can Recognize Unusual, Rare, and Unique Processed Peptide/MHC Complexes. In: Journal of Immunology. 1996 ; Vol. 157, No. 10. pp. 4464-4473.
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