Allograft Rejection by T Cell Receptor Transgenic Mice

Hamid Bassiri, James F. Markmann, Niraj M Desai, James I. Kim, Hung Sia Teh, Clyde F. Barker

Research output: Contribution to journalArticle

Abstract

We have used a line of T cell receptor (TcR) transgenic mice, in which a significant portion of CD8+ T cells expresses a TcR that is specific for the minor histocompatibility antigen H-Y presented by the H-2Db Class I molecule, to examine the immune response to H-Y-incompatible skin or pancreatic islet allografts. Our results indicate that, in contrast to the conclusions of previous reports, pancreatic islet endocrine cells are invulnerable or only weakly vulnerable to an H-Y-directed immune response. An even more unexpected finding was that unlike normal female mice of the C57BL/6 background which consistently reject male skin within a few weeks, TcR transgene+ littermates reject male skin only infrequently. Our results are consistent with the conclusion that the inability of H-Y TcR females to reject male skin is due to a deficiency of cells with male-specific helper activity. Long-term acceptance of male grafts by H-Y TcR females leads to a state of T cell hyporesponsiveness to male skin grafts. In addition, T cells harvested from long-term skin acceptors were hyporesponsive to in vitro stimulation by a clonotype-specific monoclonal antibody. Transgenic mice with TcRs having antigenic specificity for defined transplantation antigens provide a unique model for study of the allograft response.

Original languageEnglish (US)
Pages (from-to)437-444
Number of pages8
JournalJournal of Surgical Research
Volume54
Issue number5
DOIs
StatePublished - May 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Surgery

Cite this

Bassiri, H., Markmann, J. F., Desai, N. M., Kim, J. I., Teh, H. S., & Barker, C. F. (1993). Allograft Rejection by T Cell Receptor Transgenic Mice. Journal of Surgical Research, 54(5), 437-444. https://doi.org/10.1006/jsre.1993.1069