Allelotype of posterior uveal melanoma

Implications for a bifurcated tumor progression pathway

Research output: Contribution to journalArticle

Abstract

To further elucidate the somatic genetic alterations leading to acquired choroidal and ciliochoroidal melanoma, we screened every autosomal arm and the X chromosome of 50 primary posterior melanomas (31 choroidal tumors and 19 ciliochoroidal tumors). A minimum of two microsatellite markers were used to achieve at least 90% informativity (excluding X). Twenty-eight of 47 informative tumors (59%) showed allelic loss of all informative markers on chromosome 3, consistent with monosomy 3 (M3). Allelic imbalance of 8q was observed in 60% of tumors. A total of 28% of tumors displayed allelic loss of 6p. We then compared these genetic alterations with the status of chromosome 3 and found a relative absence of 6p alteration in tumors with M3 (P = 0.0005). Additionally, all observed 8q imbalance was associated with either M3 or alteration of 6p, suggesting that 8q alterations occur later in tumor progression. The mutual exclusivity of M3 and 6p alterations suggests a bifurcated tumor progression model. In this model, M3 or 6p loss identify distinct pathways, both followed by 8q loss in tumor progression.

Original languageEnglish (US)
Pages (from-to)3032-3037
Number of pages6
JournalCancer Research
Volume59
Issue number13
StatePublished - Jul 1 1999

Fingerprint

Monosomy
Neoplasms
Chromosomes, Human, Pair 3
Loss of Heterozygosity
Melanoma
Allelic Imbalance
Uveal melanoma
X Chromosome
Microsatellite Repeats

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Allelotype of posterior uveal melanoma : Implications for a bifurcated tumor progression pathway. / Parrella, Paola; Sidransky, David; Merbs, Shannath L.

In: Cancer Research, Vol. 59, No. 13, 01.07.1999, p. 3032-3037.

Research output: Contribution to journalArticle

@article{aca6adac308a4c6b847cce53c2131154,
title = "Allelotype of posterior uveal melanoma: Implications for a bifurcated tumor progression pathway",
abstract = "To further elucidate the somatic genetic alterations leading to acquired choroidal and ciliochoroidal melanoma, we screened every autosomal arm and the X chromosome of 50 primary posterior melanomas (31 choroidal tumors and 19 ciliochoroidal tumors). A minimum of two microsatellite markers were used to achieve at least 90{\%} informativity (excluding X). Twenty-eight of 47 informative tumors (59{\%}) showed allelic loss of all informative markers on chromosome 3, consistent with monosomy 3 (M3). Allelic imbalance of 8q was observed in 60{\%} of tumors. A total of 28{\%} of tumors displayed allelic loss of 6p. We then compared these genetic alterations with the status of chromosome 3 and found a relative absence of 6p alteration in tumors with M3 (P = 0.0005). Additionally, all observed 8q imbalance was associated with either M3 or alteration of 6p, suggesting that 8q alterations occur later in tumor progression. The mutual exclusivity of M3 and 6p alterations suggests a bifurcated tumor progression model. In this model, M3 or 6p loss identify distinct pathways, both followed by 8q loss in tumor progression.",
author = "Paola Parrella and David Sidransky and Merbs, {Shannath L}",
year = "1999",
month = "7",
day = "1",
language = "English (US)",
volume = "59",
pages = "3032--3037",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "13",

}

TY - JOUR

T1 - Allelotype of posterior uveal melanoma

T2 - Implications for a bifurcated tumor progression pathway

AU - Parrella, Paola

AU - Sidransky, David

AU - Merbs, Shannath L

PY - 1999/7/1

Y1 - 1999/7/1

N2 - To further elucidate the somatic genetic alterations leading to acquired choroidal and ciliochoroidal melanoma, we screened every autosomal arm and the X chromosome of 50 primary posterior melanomas (31 choroidal tumors and 19 ciliochoroidal tumors). A minimum of two microsatellite markers were used to achieve at least 90% informativity (excluding X). Twenty-eight of 47 informative tumors (59%) showed allelic loss of all informative markers on chromosome 3, consistent with monosomy 3 (M3). Allelic imbalance of 8q was observed in 60% of tumors. A total of 28% of tumors displayed allelic loss of 6p. We then compared these genetic alterations with the status of chromosome 3 and found a relative absence of 6p alteration in tumors with M3 (P = 0.0005). Additionally, all observed 8q imbalance was associated with either M3 or alteration of 6p, suggesting that 8q alterations occur later in tumor progression. The mutual exclusivity of M3 and 6p alterations suggests a bifurcated tumor progression model. In this model, M3 or 6p loss identify distinct pathways, both followed by 8q loss in tumor progression.

AB - To further elucidate the somatic genetic alterations leading to acquired choroidal and ciliochoroidal melanoma, we screened every autosomal arm and the X chromosome of 50 primary posterior melanomas (31 choroidal tumors and 19 ciliochoroidal tumors). A minimum of two microsatellite markers were used to achieve at least 90% informativity (excluding X). Twenty-eight of 47 informative tumors (59%) showed allelic loss of all informative markers on chromosome 3, consistent with monosomy 3 (M3). Allelic imbalance of 8q was observed in 60% of tumors. A total of 28% of tumors displayed allelic loss of 6p. We then compared these genetic alterations with the status of chromosome 3 and found a relative absence of 6p alteration in tumors with M3 (P = 0.0005). Additionally, all observed 8q imbalance was associated with either M3 or alteration of 6p, suggesting that 8q alterations occur later in tumor progression. The mutual exclusivity of M3 and 6p alterations suggests a bifurcated tumor progression model. In this model, M3 or 6p loss identify distinct pathways, both followed by 8q loss in tumor progression.

UR - http://www.scopus.com/inward/record.url?scp=0033168843&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033168843&partnerID=8YFLogxK

M3 - Article

VL - 59

SP - 3032

EP - 3037

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 13

ER -