All-trans-retinoic acid alters myc gene expression and inhibits in vitro progression in small cell lung cancer

G. P. Kalemkerian, R. K. Jasti, P. Celano, Barry D Nelkin, M. Mabry

Research output: Contribution to journalArticle

Abstract

Transitions between the small cell lung cancer and the non-small cell lung cancer phenotype occur during clinical tumor progression in small cell lung cancer. We have previously developed a culture model which mimics these transitions. In our model, the insertion of the v-Ha-ras oncogene into c-myc overexpressing NCI-H82 small cell lung cancer cells induces features characteristic of non-small cell lung cancer. We now report that treatment of NCI-H82 cells with 1 μM all-trans-retinoic acid resulted in decreased cellular growth, decreased c-myc mRNA levels, and increased L-myc mRNA levels. Retinoic acid treatment prior to v-Ha-ras insertion also inhibited the typical ras-induced phenotypic transition seen in untreated NCI-H82 cells. In contrast, retinoic acid treatment of NCI-H82 ras cells after ras- induced transition to the non-small cell lung cancer phenotype did not affect cellular phenotype, nor c-myc or L-myc gene expression. These data show that all-trans-retinoic acid, a clinically relevant compound, inhibits small cell lung cancer progression in our in vitro model and alters the expression of the c-myc and L-myc oncogenes. These findings suggest mechanisms for the biological effects of retinoic acid in small cell lung cancer.

Original languageEnglish (US)
Pages (from-to)55-60
Number of pages6
JournalCell Growth and Differentiation
Volume5
Issue number1
StatePublished - 1994
Externally publishedYes

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myc Genes
Small Cell Lung Carcinoma
Tretinoin
Gene Expression
Non-Small Cell Lung Carcinoma
Phenotype
Messenger RNA
ras Genes
In Vitro Techniques
Growth
Neoplasms

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

All-trans-retinoic acid alters myc gene expression and inhibits in vitro progression in small cell lung cancer. / Kalemkerian, G. P.; Jasti, R. K.; Celano, P.; Nelkin, Barry D; Mabry, M.

In: Cell Growth and Differentiation, Vol. 5, No. 1, 1994, p. 55-60.

Research output: Contribution to journalArticle

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AU - Mabry, M.

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