Alkaline phosphatase-conjugated oligonucleotide probes for enterotoxigenic Escherichia coli in travelers to South America and West Africa

J. J. Oprandy, S. A. Thornton, C. H. Gardiner, D. Burr, R. Batchelor, Louis Bourgeois

Research output: Contribution to journalArticle

Abstract

Several studies have demonstrated the usefulness of 32P-labeled recombinant DNA probes for identifying enterotoxigenic Escherichia coli (ETEC). The use of radioisotopes and X-ray development, however, severely handicaps the utility of DNA probes in most clinical laboratories. In this study, enzyme-labeled oligonucleotide probes for ETEC LT (heat-labile toxin) and ST (heat-stable toxin) genes were compared with the standard Y1 adrenal cell and suckling mouse assays for their ability to identify ETEC in a population of American adults experiencing acute episodes of diarrhea in South America and West Africa. The LT probe hybridized with 12% (64 of 529) of the E. coli colonies tested, whereas 11% (57 of 529) were positive by Y1 adrenal cell assay. DNA from 9% (47 of 529) of the E. coli colonies tested hybridized with the ST probe, whereas only 5% (28 of 529) produced ST as measured by the suckling mouse bioassay. For the patient samples tested, correlation between probe and bioassay for LT was 97%, or three discrepancies in 111 patients tested. Overall concordance of the ST probe and bioassay was 95%, or five discrepancies in 111 patients. Enzyme-labeled oligonucleotide probes represent a major advance in the diagnosis of ETEC-associated diarrheal disease and may be used in laboratories with minimal equipment.

Original languageEnglish (US)
Pages (from-to)92-95
Number of pages4
JournalJournal of Clinical Microbiology
Volume26
Issue number1
StatePublished - 1988
Externally publishedYes

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Namibia
Enterotoxigenic Escherichia coli
Oligonucleotide Probes
South America
Alkaline Phosphatase
Hot Temperature
Biological Assay
DNA Probes
Escherichia coli
Recombinant DNA
Enzymes
Radioisotopes
Diarrhea
X-Rays
Equipment and Supplies
DNA
Population
Genes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Alkaline phosphatase-conjugated oligonucleotide probes for enterotoxigenic Escherichia coli in travelers to South America and West Africa. / Oprandy, J. J.; Thornton, S. A.; Gardiner, C. H.; Burr, D.; Batchelor, R.; Bourgeois, Louis.

In: Journal of Clinical Microbiology, Vol. 26, No. 1, 1988, p. 92-95.

Research output: Contribution to journalArticle

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