Alignment of caldesmon on the actin-tropomyosin filaments

T. S. Tsuruda, M. H. Watson, Darren Brian Foster, J. J C Lin, A. S. Mak

Research output: Contribution to journalArticle

Abstract

We have reported previously tat each smooth-muscle caldesmon binds predominately to a region within residues 142-227 of tropomyosin, but a weaker binding site also exists at the N-terminal region of tropomyosin. In view of recent evidence for the presence of tropomyosin-binding sites at both the N- and C-terminal domains of caldesmon, we have studied the binding of the N- and C-terminal fragments of human fibroblast caldesmon expressed in Escherichia coli to tropomyosin and its CNBr fragments. The N-terminal fragment, CaD40 (residues 1-152), binds tropomoysin, but the interaction is mostly abolished in the presence of actin. CaD40 binds strongly to Cn1B(142-281) of tropomoysin, but weakly to Cn1A(11-127). The C-terminal fragment, CaD39, which corresponds to residues 443-736 of gizzard caldesmon, binds tropomoysin, and the interaction is enhanced by actin. CaD39 binds to both Cn1A(11-127) and Cn1B(142-281) of tropomyosin Our results suggest that the N-terminal domain of caldesmon interacts with the C-terminal half of one tropomyosin molecule, whereas the C-terminal domain binds to both N- and C-terminal regions of the adjacent tropomyosin molecule along the actin filament. In addition, the binding of the N- terminal domain of caldesmon to the actin-tropomyosin filament is weak, which may allow this domain to project off the thin filament to interact with myosin.

Original languageEnglish (US)
Pages (from-to)951-957
Number of pages7
JournalBiochemical Journal
Volume309
Issue number3
StatePublished - 1995
Externally publishedYes

Fingerprint

Calmodulin-Binding Proteins
Tropomyosin
Actin Cytoskeleton
Actins
Binding Sites
Molecules
Fibroblasts
Myosins
Escherichia coli
Smooth Muscle
Muscle

ASJC Scopus subject areas

  • Biochemistry

Cite this

Tsuruda, T. S., Watson, M. H., Foster, D. B., Lin, J. J. C., & Mak, A. S. (1995). Alignment of caldesmon on the actin-tropomyosin filaments. Biochemical Journal, 309(3), 951-957.

Alignment of caldesmon on the actin-tropomyosin filaments. / Tsuruda, T. S.; Watson, M. H.; Foster, Darren Brian; Lin, J. J C; Mak, A. S.

In: Biochemical Journal, Vol. 309, No. 3, 1995, p. 951-957.

Research output: Contribution to journalArticle

Tsuruda, TS, Watson, MH, Foster, DB, Lin, JJC & Mak, AS 1995, 'Alignment of caldesmon on the actin-tropomyosin filaments', Biochemical Journal, vol. 309, no. 3, pp. 951-957.
Tsuruda TS, Watson MH, Foster DB, Lin JJC, Mak AS. Alignment of caldesmon on the actin-tropomyosin filaments. Biochemical Journal. 1995;309(3):951-957.
Tsuruda, T. S. ; Watson, M. H. ; Foster, Darren Brian ; Lin, J. J C ; Mak, A. S. / Alignment of caldesmon on the actin-tropomyosin filaments. In: Biochemical Journal. 1995 ; Vol. 309, No. 3. pp. 951-957.
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