Abstract
This unit shows how to use the Bowtie package to align short sequencing reads, such as those output by second-generation sequencing instruments. It also includes protocols for building a genome index and calling consensus sequences from Bowtie alignments using SAMtools.
| Original language | English (US) |
|---|---|
| Article number | 11.7 |
| Journal | Current Protocols in Bioinformatics |
| Issue number | SUPP.32 |
| DOIs | |
| State | Published - Dec 2010 |
| Externally published | Yes |
Keywords
- Alignment
- Comparative genomics
- Genome indexing
- Mapping
- Read alignment
- Read mapping
- Short reads
- Software package
ASJC Scopus subject areas
- Structural Biology
- Biochemistry