TY - JOUR
T1 - Aldosterone-mediated Na/K-ATPase expression is α1 isoform specific in the renal cortical collecting duct
AU - Welling, Paul A.
AU - Caplan, Michael
AU - Sutters, Michael
AU - Giebisch, Gerhard
PY - 1993/11/5
Y1 - 1993/11/5
N2 - In the renal cortical collecting duct (CCD), mineralocorticoid hormones, like aldosterone, augment the abundance of Na/K-ATPase molecules. It has been postulated that this response involves an isoform switch of the Na/K-ATPase catalytic subunit, α, as the molecular basis for the differential regulation of mineralocorticoid-induced and constitutively expressed Na/K-ATPase pools. In opposition to this attractive hypothesis, three lines of independent evidence are presented which demonstrate that the CCD exclusively expresses the α1 form despite mineralocorticoid-mediated changes in functional Na/K pump density. First, aldosterone increased [3H]ouabain binding in CCD 2.5-fold without changing the ouabain dissociation constant. Second, an electrophysiological assay for pump activity revealed that aldosterone increased maximum Na/ K pump current in parallel with the change in ouabain binding without altering the apparent sodium affinity. Third, Western blot analysis with α isoform-specific, antipeptide antibodies demonstrated that aldosterone exclusively increased the total chemical pool of the on form of the pump without inducing other α subunit isoforms. In summary, aldosterone increases the abundance of Na/K-ATPase molecules in the CCD which are pharmacologically, physiologically, and chemically indistinguishable from those that are normally expressed.
AB - In the renal cortical collecting duct (CCD), mineralocorticoid hormones, like aldosterone, augment the abundance of Na/K-ATPase molecules. It has been postulated that this response involves an isoform switch of the Na/K-ATPase catalytic subunit, α, as the molecular basis for the differential regulation of mineralocorticoid-induced and constitutively expressed Na/K-ATPase pools. In opposition to this attractive hypothesis, three lines of independent evidence are presented which demonstrate that the CCD exclusively expresses the α1 form despite mineralocorticoid-mediated changes in functional Na/K pump density. First, aldosterone increased [3H]ouabain binding in CCD 2.5-fold without changing the ouabain dissociation constant. Second, an electrophysiological assay for pump activity revealed that aldosterone increased maximum Na/ K pump current in parallel with the change in ouabain binding without altering the apparent sodium affinity. Third, Western blot analysis with α isoform-specific, antipeptide antibodies demonstrated that aldosterone exclusively increased the total chemical pool of the on form of the pump without inducing other α subunit isoforms. In summary, aldosterone increases the abundance of Na/K-ATPase molecules in the CCD which are pharmacologically, physiologically, and chemically indistinguishable from those that are normally expressed.
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M3 - Article
C2 - 8226873
AN - SCOPUS:0027505017
SN - 0021-9258
VL - 268
SP - 23469
EP - 23476
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 31
ER -