Alcohol dehydrogenase activity in the uremic rat

Esteban Mezey; James J. Potter

doi:10.1016/0024-3205(78)90543-X

Alcohol dehydrogenase activity in the uremic rat

Esteban Mezey, James J. Potter

School of Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

An increase in liver alcohol dehydrogenase activity was detected after 6 days of uremia in the rat, with a maximum increase occurring after 10 days. The increase was documented in the oxidative and reductive directions with ethanol and acetaldehyde as substrates, respectively; was not found in organs other than the liver and was not accompanied by increases in other cytosolic enzymes. The enzyme in the uremic animal did not differ in pH optimum for ethanol oxidation, K_m for ethanol and NAD⁺ or in electrophoretic mobility from the enzyme in the normal animal. Adrenalectomy prevented the increase. It is suggested that the increase in liver alcohol dehydrogenase requires intact adrenal glands and is most likely caused by the stress of surgery and the uremic state.

Original language	English (US)
Pages (from-to)	1985-1991
Number of pages	7
Journal	Life Sciences
Volume	22
Issue number	22
DOIs	https://doi.org/10.1016/0024-3205(78)90543-X
State	Published - Jun 12 1978

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(78)90543-X

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title = "Alcohol dehydrogenase activity in the uremic rat",

abstract = "An increase in liver alcohol dehydrogenase activity was detected after 6 days of uremia in the rat, with a maximum increase occurring after 10 days. The increase was documented in the oxidative and reductive directions with ethanol and acetaldehyde as substrates, respectively; was not found in organs other than the liver and was not accompanied by increases in other cytosolic enzymes. The enzyme in the uremic animal did not differ in pH optimum for ethanol oxidation, Km for ethanol and NAD+ or in electrophoretic mobility from the enzyme in the normal animal. Adrenalectomy prevented the increase. It is suggested that the increase in liver alcohol dehydrogenase requires intact adrenal glands and is most likely caused by the stress of surgery and the uremic state.",

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N2 - An increase in liver alcohol dehydrogenase activity was detected after 6 days of uremia in the rat, with a maximum increase occurring after 10 days. The increase was documented in the oxidative and reductive directions with ethanol and acetaldehyde as substrates, respectively; was not found in organs other than the liver and was not accompanied by increases in other cytosolic enzymes. The enzyme in the uremic animal did not differ in pH optimum for ethanol oxidation, Km for ethanol and NAD+ or in electrophoretic mobility from the enzyme in the normal animal. Adrenalectomy prevented the increase. It is suggested that the increase in liver alcohol dehydrogenase requires intact adrenal glands and is most likely caused by the stress of surgery and the uremic state.

AB - An increase in liver alcohol dehydrogenase activity was detected after 6 days of uremia in the rat, with a maximum increase occurring after 10 days. The increase was documented in the oxidative and reductive directions with ethanol and acetaldehyde as substrates, respectively; was not found in organs other than the liver and was not accompanied by increases in other cytosolic enzymes. The enzyme in the uremic animal did not differ in pH optimum for ethanol oxidation, Km for ethanol and NAD+ or in electrophoretic mobility from the enzyme in the normal animal. Adrenalectomy prevented the increase. It is suggested that the increase in liver alcohol dehydrogenase requires intact adrenal glands and is most likely caused by the stress of surgery and the uremic state.

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JO - Life Sciences

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ER -

Alcohol dehydrogenase activity in the uremic rat

Abstract

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