Akt signaling dynamics in plasma membrane microdomains visualized by FRET-based reporters

Xinxin Gao, Jin Zhang

Research output: Contribution to journalArticle

Abstract

Human kinase Akt has been shown to be activated at the plasma membrane upon generation of the cell membrane bound second messenger phosphatidylinositol (3,4,5)-trisphosphate through phosphatidylinositol 3-kinase (PI3K). Several components in the PI3K/Akt signaling pathway have been found in the detergentresistant plasma membrane compartments, lipid rafts. Increasing evidence also suggests crucial roles of lipid rafts in the activation of Akt in different cell types. However, the regulatory mechanisms of Akt activation at different microdomains of the plasma membrane are not clear. Using a newly developed genetically encodable fluorescent kinase reporter based on fluorescence resonance energy transfer (FRET), AktAR, we studied spatio-temporal dynamics of Akt activity within plasma membrane microdomains in live-cell context. Our studies suggest that Akt activity is turned on more rapidly in lipid rafts upon growth factor stimulation, and plateletderived growth factor (PDGF) or insulin-like Growth Factor-1 (IGF-1) stimulated Akt activity is differentially regulated between raft and non-raft regions of the plasma membrane.

Original languageEnglish (US)
Pages (from-to)32-34
Number of pages3
JournalCommunicative and Integrative Biology
Volume2
Issue number1
StatePublished - Jan 2009

Fingerprint

Membrane Microdomains
Fluorescence Resonance Energy Transfer
energy transfer
plasma membrane
Cell Membrane
fluorescence
Phosphatidylinositol 3-Kinase
phosphatidylinositol 3-kinase
growth factors
phosphotransferases (kinases)
lipids
Intercellular Signaling Peptides and Proteins
Phosphotransferases
second messengers
phosphatidylinositols
Lipids
somatomedins
Second Messenger Systems
Somatomedins
cell membranes

Keywords

  • Akt
  • Cholesterol
  • FRET
  • IGF-1
  • Lipid rafts
  • PDGF

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Akt signaling dynamics in plasma membrane microdomains visualized by FRET-based reporters. / Gao, Xinxin; Zhang, Jin.

In: Communicative and Integrative Biology, Vol. 2, No. 1, 01.2009, p. 32-34.

Research output: Contribution to journalArticle

@article{72b5c9f459204acc8eb65c4125cc85c8,
title = "Akt signaling dynamics in plasma membrane microdomains visualized by FRET-based reporters",
abstract = "Human kinase Akt has been shown to be activated at the plasma membrane upon generation of the cell membrane bound second messenger phosphatidylinositol (3,4,5)-trisphosphate through phosphatidylinositol 3-kinase (PI3K). Several components in the PI3K/Akt signaling pathway have been found in the detergentresistant plasma membrane compartments, lipid rafts. Increasing evidence also suggests crucial roles of lipid rafts in the activation of Akt in different cell types. However, the regulatory mechanisms of Akt activation at different microdomains of the plasma membrane are not clear. Using a newly developed genetically encodable fluorescent kinase reporter based on fluorescence resonance energy transfer (FRET), AktAR, we studied spatio-temporal dynamics of Akt activity within plasma membrane microdomains in live-cell context. Our studies suggest that Akt activity is turned on more rapidly in lipid rafts upon growth factor stimulation, and plateletderived growth factor (PDGF) or insulin-like Growth Factor-1 (IGF-1) stimulated Akt activity is differentially regulated between raft and non-raft regions of the plasma membrane.",
keywords = "Akt, Cholesterol, FRET, IGF-1, Lipid rafts, PDGF",
author = "Xinxin Gao and Jin Zhang",
year = "2009",
month = "1",
language = "English (US)",
volume = "2",
pages = "32--34",
journal = "Communicative and Integrative Biology",
issn = "1942-0889",
publisher = "Landes Bioscience",
number = "1",

}

TY - JOUR

T1 - Akt signaling dynamics in plasma membrane microdomains visualized by FRET-based reporters

AU - Gao, Xinxin

AU - Zhang, Jin

PY - 2009/1

Y1 - 2009/1

N2 - Human kinase Akt has been shown to be activated at the plasma membrane upon generation of the cell membrane bound second messenger phosphatidylinositol (3,4,5)-trisphosphate through phosphatidylinositol 3-kinase (PI3K). Several components in the PI3K/Akt signaling pathway have been found in the detergentresistant plasma membrane compartments, lipid rafts. Increasing evidence also suggests crucial roles of lipid rafts in the activation of Akt in different cell types. However, the regulatory mechanisms of Akt activation at different microdomains of the plasma membrane are not clear. Using a newly developed genetically encodable fluorescent kinase reporter based on fluorescence resonance energy transfer (FRET), AktAR, we studied spatio-temporal dynamics of Akt activity within plasma membrane microdomains in live-cell context. Our studies suggest that Akt activity is turned on more rapidly in lipid rafts upon growth factor stimulation, and plateletderived growth factor (PDGF) or insulin-like Growth Factor-1 (IGF-1) stimulated Akt activity is differentially regulated between raft and non-raft regions of the plasma membrane.

AB - Human kinase Akt has been shown to be activated at the plasma membrane upon generation of the cell membrane bound second messenger phosphatidylinositol (3,4,5)-trisphosphate through phosphatidylinositol 3-kinase (PI3K). Several components in the PI3K/Akt signaling pathway have been found in the detergentresistant plasma membrane compartments, lipid rafts. Increasing evidence also suggests crucial roles of lipid rafts in the activation of Akt in different cell types. However, the regulatory mechanisms of Akt activation at different microdomains of the plasma membrane are not clear. Using a newly developed genetically encodable fluorescent kinase reporter based on fluorescence resonance energy transfer (FRET), AktAR, we studied spatio-temporal dynamics of Akt activity within plasma membrane microdomains in live-cell context. Our studies suggest that Akt activity is turned on more rapidly in lipid rafts upon growth factor stimulation, and plateletderived growth factor (PDGF) or insulin-like Growth Factor-1 (IGF-1) stimulated Akt activity is differentially regulated between raft and non-raft regions of the plasma membrane.

KW - Akt

KW - Cholesterol

KW - FRET

KW - IGF-1

KW - Lipid rafts

KW - PDGF

UR - http://www.scopus.com/inward/record.url?scp=60949097086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60949097086&partnerID=8YFLogxK

M3 - Article

C2 - 19704863

AN - SCOPUS:60949097086

VL - 2

SP - 32

EP - 34

JO - Communicative and Integrative Biology

JF - Communicative and Integrative Biology

SN - 1942-0889

IS - 1

ER -