TY - JOUR
T1 - Airway narrowing in healthy humans inhaling methacholine without deep inspirations demonstrated by HRCT
AU - Brown, Robert H.
AU - Croisille, Pierre
AU - Mudge, Beatrice
AU - Diemer, Fiona B.
AU - Permutt, Solbert
AU - Togias, Alkis
PY - 2000
Y1 - 2000
N2 - Normal subjects prevented from taking a deep breath show changes in airflow similar to those of asthmatics when challenged with methacholine (MCh). To confirm airway narrowing by MCh in this setting and to determine its location, we concurrently measured changes in airway lumenal area using high resolution computed tomography (HRCT) and airflow using partial spirometry in five normal subjects challenged with increasing doses of MCh under prohibition of deep breaths. In an attempt to improve imaging accuracy, we corrected for the changes in lung volume during bronchoprovocation. At every step of the provocation, scanning was performed at approximately the same lung volume. On the HRCT images, airway area decreased in response to the increasing doses of MCh to 91 (+) 2%, 88 (+) 2%, and 80 (+) 2% of baseline at the doses of MCh 0.25, 0.75, and 2.5 mg/ml, respectively (p < 0.001). Airway narrowing showed no predilection for particular airway sizes and occurred in a heterogeneous pattern. The changes in the mean airway lumenal area as measured by HRCT and the mean partial spirometric outcomes were highly correlated: FEV1p (r2 = 0.46, p = 0.001), FVCp (r2 = 0.20, p = 0.05), FEV1/FVCp (r2 = 0.55, p = 0.002), MMEFp (r2 = 0.31, p = 0.01), and τp (r2 = 0.51, p = 0.0004). We conclude that in normal subjects who are prevented from taking a deep breath, the spirometric changes occurring with aerosol MCh challenge are associated with conducting airway narrowing.
AB - Normal subjects prevented from taking a deep breath show changes in airflow similar to those of asthmatics when challenged with methacholine (MCh). To confirm airway narrowing by MCh in this setting and to determine its location, we concurrently measured changes in airway lumenal area using high resolution computed tomography (HRCT) and airflow using partial spirometry in five normal subjects challenged with increasing doses of MCh under prohibition of deep breaths. In an attempt to improve imaging accuracy, we corrected for the changes in lung volume during bronchoprovocation. At every step of the provocation, scanning was performed at approximately the same lung volume. On the HRCT images, airway area decreased in response to the increasing doses of MCh to 91 (+) 2%, 88 (+) 2%, and 80 (+) 2% of baseline at the doses of MCh 0.25, 0.75, and 2.5 mg/ml, respectively (p < 0.001). Airway narrowing showed no predilection for particular airway sizes and occurred in a heterogeneous pattern. The changes in the mean airway lumenal area as measured by HRCT and the mean partial spirometric outcomes were highly correlated: FEV1p (r2 = 0.46, p = 0.001), FVCp (r2 = 0.20, p = 0.05), FEV1/FVCp (r2 = 0.55, p = 0.002), MMEFp (r2 = 0.31, p = 0.01), and τp (r2 = 0.51, p = 0.0004). We conclude that in normal subjects who are prevented from taking a deep breath, the spirometric changes occurring with aerosol MCh challenge are associated with conducting airway narrowing.
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U2 - 10.1164/ajrccm.161.4.9806051
DO - 10.1164/ajrccm.161.4.9806051
M3 - Article
C2 - 10764321
AN - SCOPUS:0034057271
SN - 1073-449X
VL - 161
SP - 1256
EP - 1263
JO - American journal of respiratory and critical care medicine
JF - American journal of respiratory and critical care medicine
IS - 4 I
ER -