Airway mucosal host defense is key to genomic regulation of cystic fibrosis lung disease severity

Deepika Polineni, Hong Dang, Paul J. Gallins, Lisa C. Jones, Rhonda G. Pace, Jaclyn R. Stonebraker, Leah A. Commander, Jeanne E. Krenicky, Yi Hui Zhou, Harriet Corvol, Garry R. Cutting, Mitchell L. Drumm, Lisa J. Strug, Michael P Boyle, Peter R. Durie, James F. Chmiel, Fei Zou, Fred A. Wright, Wanda K. O’Neal, Michael R. Knowles

Research output: Contribution to journalArticle

Abstract

Rationale: The severity of cystic fibrosis (CF) lung disease varies widely, even for Phe508del homozygotes. Heritability studies show that more than 50% of the variability reflects non-cystic fibrosis transmembrane conductance regulator (CFTR) genetic variation; however, the full extent of the pertinent genetic variation is not known. Objectives: We sought to identify novel CF disease-modifying mechanisms using an integrated approach based on analyzing “in vivo” CF airway epithelial gene expression complemented with genome-wide association study (GWAS) data. Methods: Nasal mucosal RNA from 134 patients with CF was used for RNA sequencing. We tested for associations of transcriptomic (gene expression) data with a quantitative phenotype of CF lung disease severity. Pathway analysis of CF GWAS data (n = 5,659 patients) was performed to identify novel pathways and assess the concordance of genomic and transcriptomic data. Association of gene expression with previously identified CF GWAS risk alleles was also tested. Measurements and Main Results: Significant evidence of heritable gene expression was identified. Gene expression pathways relevant to airway mucosal host defense were significantly associated with CF lung disease severity, including viral infection, inflammation/inflammatory signaling, lipid metabolism, apoptosis, ion transport, Phe508del CFTR processing, and innate immune responses, including HLA (human leukocyte antigen) genes. Ion transport and CFTR processing pathways, as well as HLA genes, were identified across differential gene expression and GWAS signals. Conclusions: Transcriptomic analyses of CF airway epithelia, coupled to genomic (GWAS) analyses, highlight the role of heritable host defense variation in determining the pathophysiology of CF lung disease. The identification of these pathways provides opportunities to pursue targeted interventions to improve CF lung health.

Original languageEnglish (US)
Pages (from-to)79-93
Number of pages15
JournalAmerican journal of respiratory and critical care medicine
Volume197
Issue number1
DOIs
StatePublished - Jan 1 2018

Keywords

  • Cystic fibrosis
  • Epithelia
  • Genome
  • Genome-wide association study
  • Transcriptome

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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    Polineni, D., Dang, H., Gallins, P. J., Jones, L. C., Pace, R. G., Stonebraker, J. R., Commander, L. A., Krenicky, J. E., Zhou, Y. H., Corvol, H., Cutting, G. R., Drumm, M. L., Strug, L. J., Boyle, M. P., Durie, P. R., Chmiel, J. F., Zou, F., Wright, F. A., O’Neal, W. K., & Knowles, M. R. (2018). Airway mucosal host defense is key to genomic regulation of cystic fibrosis lung disease severity. American journal of respiratory and critical care medicine, 197(1), 79-93. https://doi.org/10.1164/rccm.201701-0134OC