Agrin induces phosphorylation of the nicotinic acetylcholine receptor

Bruce G. Wallace, Qu Zhican, Huganir L. Richard

Research output: Contribution to journalArticlepeer-review

Abstract

Agrin causes acetylcholine receptors (AChRs) on chick myotubes in culture to aggregate, forming specializations that resemble the postsynaptic apparatus at the vertebrate skeletal neuromuscular junction. Here we report that treating chick myotubes with agrin caused an increase in phosphorylation of the AChR β, γ, and δ subunits. H-7, a potent inhibitor of several protein serine kinases, blocked agrin-induced phosphorylation of the γ and δ subunits, but did not prevent either agrin-induced AChR aggregation or phosphorylation of the β subunit. Experiments with anti-phosphotyrosine antibodies demonstrated that agrin caused an increase in tyrosine phosphorylation of the β subunit that began within 30 min of adding agrin to the myotube cultures, reached a plateau by 3 hr, and was blocked by treatments known to block agrin-induced AChR aggregation. Anti-phosphotyrosine antibodies labeled agrin-induced specializations as they do the postsynaptic apparatus. These results suggest that agrin-induced tyrosine phosphorylation of the β subunit may play a role in regulating AChR distribution.

Original languageEnglish (US)
Pages (from-to)869-878
Number of pages10
JournalNeuron
Volume6
Issue number6
DOIs
StatePublished - Jun 1991

ASJC Scopus subject areas

  • Neuroscience(all)

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