Aging effects of vitamin C on a human lens protein produced in vitro

P. Russell, D. Garland, J. S. Zigler, S. O. Meakin, L. C. Tsui, M. L. Breitman

Research output: Contribution to journalArticle

Abstract

Human lens γ-crystallin obtained from the expression of a gene construct stably integraged into mouse L cells was incubated with ascorbate in the presence of iron and oxygen. The resulting oxidation of the γ-crystallin led to more acidic species of this protein. These alterations were similar to the changes seen with aging in the human lens. The results suggest that oxidation of lens crystallins may responsible for the changes seen on aging and cataract development and that ascorbate may contribute to these alterations.

Original languageEnglish (US)
Pages (from-to)32-35
Number of pages4
JournalFASEB Journal
Volume1
Issue number1
StatePublished - 1987
Externally publishedYes

Fingerprint

crystallins
Crystallins
Lens
Lenses
Ascorbic Acid
Aging of materials
ascorbic acid
oxidation
Oxidation
proteins
cataract
Cataract
Iron
Genes
iron
Oxygen
oxygen
Gene Expression
gene expression
mice

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Russell, P., Garland, D., Zigler, J. S., Meakin, S. O., Tsui, L. C., & Breitman, M. L. (1987). Aging effects of vitamin C on a human lens protein produced in vitro. FASEB Journal, 1(1), 32-35.

Aging effects of vitamin C on a human lens protein produced in vitro. / Russell, P.; Garland, D.; Zigler, J. S.; Meakin, S. O.; Tsui, L. C.; Breitman, M. L.

In: FASEB Journal, Vol. 1, No. 1, 1987, p. 32-35.

Research output: Contribution to journalArticle

Russell, P, Garland, D, Zigler, JS, Meakin, SO, Tsui, LC & Breitman, ML 1987, 'Aging effects of vitamin C on a human lens protein produced in vitro', FASEB Journal, vol. 1, no. 1, pp. 32-35.
Russell P, Garland D, Zigler JS, Meakin SO, Tsui LC, Breitman ML. Aging effects of vitamin C on a human lens protein produced in vitro. FASEB Journal. 1987;1(1):32-35.
Russell, P. ; Garland, D. ; Zigler, J. S. ; Meakin, S. O. ; Tsui, L. C. ; Breitman, M. L. / Aging effects of vitamin C on a human lens protein produced in vitro. In: FASEB Journal. 1987 ; Vol. 1, No. 1. pp. 32-35.
@article{8745b9f178ab419abc053b26894f0183,
title = "Aging effects of vitamin C on a human lens protein produced in vitro",
abstract = "Human lens γ-crystallin obtained from the expression of a gene construct stably integraged into mouse L cells was incubated with ascorbate in the presence of iron and oxygen. The resulting oxidation of the γ-crystallin led to more acidic species of this protein. These alterations were similar to the changes seen with aging in the human lens. The results suggest that oxidation of lens crystallins may responsible for the changes seen on aging and cataract development and that ascorbate may contribute to these alterations.",
author = "P. Russell and D. Garland and Zigler, {J. S.} and Meakin, {S. O.} and Tsui, {L. C.} and Breitman, {M. L.}",
year = "1987",
language = "English (US)",
volume = "1",
pages = "32--35",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "1",

}

TY - JOUR

T1 - Aging effects of vitamin C on a human lens protein produced in vitro

AU - Russell, P.

AU - Garland, D.

AU - Zigler, J. S.

AU - Meakin, S. O.

AU - Tsui, L. C.

AU - Breitman, M. L.

PY - 1987

Y1 - 1987

N2 - Human lens γ-crystallin obtained from the expression of a gene construct stably integraged into mouse L cells was incubated with ascorbate in the presence of iron and oxygen. The resulting oxidation of the γ-crystallin led to more acidic species of this protein. These alterations were similar to the changes seen with aging in the human lens. The results suggest that oxidation of lens crystallins may responsible for the changes seen on aging and cataract development and that ascorbate may contribute to these alterations.

AB - Human lens γ-crystallin obtained from the expression of a gene construct stably integraged into mouse L cells was incubated with ascorbate in the presence of iron and oxygen. The resulting oxidation of the γ-crystallin led to more acidic species of this protein. These alterations were similar to the changes seen with aging in the human lens. The results suggest that oxidation of lens crystallins may responsible for the changes seen on aging and cataract development and that ascorbate may contribute to these alterations.

UR - http://www.scopus.com/inward/record.url?scp=0023607180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023607180&partnerID=8YFLogxK

M3 - Article

VL - 1

SP - 32

EP - 35

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 1

ER -