Aging effects of vitamin C on a human lens protein produced in vitro

P. Russell, D. Garland, J. S. Zigler, S. O. Meakin, L. C. Tsui, M. L. Breitman

Research output: Contribution to journalArticlepeer-review

Abstract

Human lens γ-crystallin obtained from the expression of a gene construct stably integraged into mouse L cells was incubated with ascorbate in the presence of iron and oxygen. The resulting oxidation of the γ-crystallin led to more acidic species of this protein. These alterations were similar to the changes seen with aging in the human lens. The results suggest that oxidation of lens crystallins may responsible for the changes seen on aging and cataract development and that ascorbate may contribute to these alterations.

Original languageEnglish (US)
Pages (from-to)32-35
Number of pages4
JournalFASEB Journal
Volume1
Issue number1
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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