Aging effects of vitamin C on a human lens protein produced in vitro

P. Russell; D. Garland; J. S. Zigler; S. O. Meakin; L. C. Tsui; M. L. Breitman

doi:10.1096/fasebj.1.1.3301496

Aging effects of vitamin C on a human lens protein produced in vitro

P. Russell, D. Garland, J. S. Zigler, S. O. Meakin, L. C. Tsui, M. L. Breitman

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Human lens γ-crystallin obtained from the expression of a gene construct stably integraged into mouse L cells was incubated with ascorbate in the presence of iron and oxygen. The resulting oxidation of the γ-crystallin led to more acidic species of this protein. These alterations were similar to the changes seen with aging in the human lens. The results suggest that oxidation of lens crystallins may responsible for the changes seen on aging and cataract development and that ascorbate may contribute to these alterations.

Original language	English (US)
Pages (from-to)	32-35
Number of pages	4
Journal	FASEB Journal
Volume	1
Issue number	1
DOIs	https://doi.org/10.1096/fasebj.1.1.3301496
State	Published - 1987
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Genetics

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AU - Garland, D.

AU - Zigler, J. S.

AU - Meakin, S. O.

AU - Tsui, L. C.

AU - Breitman, M. L.

PY - 1987

Y1 - 1987

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AB - Human lens γ-crystallin obtained from the expression of a gene construct stably integraged into mouse L cells was incubated with ascorbate in the presence of iron and oxygen. The resulting oxidation of the γ-crystallin led to more acidic species of this protein. These alterations were similar to the changes seen with aging in the human lens. The results suggest that oxidation of lens crystallins may responsible for the changes seen on aging and cataract development and that ascorbate may contribute to these alterations.

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Aging effects of vitamin C on a human lens protein produced in vitro

Abstract

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