Aggregate formation of erythrocytes in postcapillary venules

Sangho Kim, Aleksander S Popel, Marcos Intaglietta, Paul C. Johnson

Research output: Contribution to journalArticle

Abstract

The purpose of the present study was to obtain information on erythrocyte aggregate formation in vivo. The movements of erythrocytes in postcapillary venules of the rat spinotrapezius muscle at various flow rates were recorded with a high-speed video camera before and after infusion of dextran 500. To distinguish aggregates, the following criteria were used: 1) a fixed distance (4 μm) between the center points of two adjacent cells, 2) lack of visible separation between the adjacent cells, and 3) movement of the adjacent cells in the same direction. Without dextran 500 infusion, 11 and 5% of erythrocytes formed aggregates in low (33.2 ± 28.3 s) and high pseudoshear (144.2 ± 58.3 s) conditions, respectively, based on the above criteria. After dextran 500 infusion, 53% of erythrocytes satisfied the criteria in the low pseudoshear condition (26.5 ± 17.0 s) and 13% of erythrocytes met the criteria in the high pseudoshear condition (240.0 ± 85.9 s), indicating erythrocyte aggregation is strongly associated with shear rate. Approximately 90% of aggregate formation occurred in a short time period (0.15-0.30 s after entering the venule) in a region 15 to 30 μm from the entrance. The time delay may reflect rheological entrance conditions in the venule.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume288
Issue number2 57-2
DOIs
StatePublished - Feb 2005

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Venules
Erythrocytes
Dextrans
Erythrocyte Aggregation
Cell Movement
Muscles

Keywords

  • Hemodynamics
  • In vivo blood rheology
  • In vivo microscopy

ASJC Scopus subject areas

  • Physiology

Cite this

Aggregate formation of erythrocytes in postcapillary venules. / Kim, Sangho; Popel, Aleksander S; Intaglietta, Marcos; Johnson, Paul C.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 288, No. 2 57-2, 02.2005.

Research output: Contribution to journalArticle

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