Aggravating genetic interactions allow a solution to redundancy in a bacterial pathogen

Tamara J. O'Connor, Dana Boyd, Marion S. Dorer, Ralph R. Isberg

Research output: Contribution to journalArticle

Abstract

Interactions between hosts and pathogens are complex, so understanding the events that govern these interactions requires the analysis of molecular mechanisms operating in both organisms. Many pathogens use multiple strategies to target a single event in the disease process, confounding the identification of the important determinants of virulence. We developed a genetic screening strategy called insertional mutagenesis and depletion (iMAD) that combines bacterial mutagenesis and RNA interference, to systematically dissect the interplay between a pathogen and its host. We used this technique to resolve the network of proteins secreted by the bacterium Legionella pneumophila to promote intracellular growth, a critical determinant of pathogenicity of this organism. This strategy is broadly applicable, allowing the dissection of any interface between two organisms involving numerous interactions.

Original languageEnglish (US)
Pages (from-to)1440-1444
Number of pages5
JournalScience
Volume338
Issue number6113
DOIs
StatePublished - Dec 14 2012
Externally publishedYes

ASJC Scopus subject areas

  • General

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