Age-Related Disease Association of Endogenous γ-H2AX Foci in Mononuclear Cells Derived from Leukapheresis

Shepherd H. Schurman, Christopher A. Dunn, Rebecca Greaves, Binbing Yu, Luigi Ferrucci, Deborah L. Croteau, Michael M. Seidman, Vilhelm A. Bohr

Research output: Contribution to journalArticlepeer-review

Abstract

The phosphorylated form of histone H2AX (γ-H2AX) forms immunohistochemically detectable foci at DNA double strand breaks. In peripheral blood mononuclear cells (PBMCs) derived from leukapheresis from patients enrolled in the Baltimore Longitudinal Study of Aging, γ-H2AX foci increased in a linear fashion with regards to age, peaking at ~57 years. The relationship between the frequency of γ-H2AX foci and age-related pathologies was assessed. We found a statistically significant (p = 0.023) 50% increase in foci in PBMCs derived from patients with a known history of vitamin D deficiency. In addition, there were trends toward increased γ-H2AX foci in patients with cataracts (34% increase, p

Original languageEnglish (US)
Article numbere45728
JournalPLoS One
Volume7
Issue number9
DOIs
StatePublished - Sep 21 2012
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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