Age-related changes in the mechanisms of LHRH-stimulated LH release from pituitary cells in vitro

Atsushi Miyamoto, Toshio Maki, Marc R. Blackman, George S. Roth

Research output: Contribution to journalArticlepeer-review

Abstract

In vitro release of LH in response to LHRH, phorbol myristate acetate (PMA), the ionophore A23187, and nifedipine was evaluated in primary cultures of anterior pituitary cells from intact mature (6 to 7 month) and old (23 to 24 month) male Wistar rats. LH release from pituitary cells is reduced approximately 30% and 60% after 4 and 48 h of 10-7M LHRH stimulation in cells of old rats, respectively. This impairment may be secondary to a loss of LHRH receptors. LHRH-stimulated LH release from cells of mature rats was inhibited 70% by the voltage-gated calcium channel blocker, nifedipine (10-6M), whereas LHRH-stimulated LH release from cells of old rat was too low to detect the effects of this drug. Age changes can be partially reversed by A23187 and PMA during 4 h, but not 48 hrs of stimulation. It therefore appears that short- and long-term (4 h and 48 h, respectively) stimulation of LH release may proceed through separate mechanisms that are differentially affected by aging.

Original languageEnglish (US)
Pages (from-to)211-219
Number of pages9
JournalExperimental Gerontology
Volume27
Issue number2
DOIs
StatePublished - 1992

Keywords

  • LH release
  • aging
  • pituitary cells

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

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