Age-related changes in tetrahydrobiopterin and GTP-cyclohydrolase activity in the brain and adrenal gland of rats

Mir Ahamed Hossain, Joseph M. Masserano, Norman Weiner

Research output: Contribution to journalArticle

Abstract

In the present study, we observed that tetrahydrobiopterin (BH4) levels were decreased significantly in the striatum, substantia nigra, frontal cortex, hypothalamus, and cerebellum of 25-month-old and in the cerebellum only in case of 18-month-old rats as compared to 4-month-old rats. In contrast, BH4 levels in adrenal glands of 25-month-old rats were increased significantly. Kinetic analysis of GTP-cyclohydrolase revealed a decrease in the apparent Km along with an increase in Vmax value in the adrenal of 25-month-old rats compared to 4-month-old rats. Whereas, in cerebellum we observed that the apparent Km of the high affinity form of the enzyme was increased and a decrease in the Vmax value of the low affinity form only in case of 25-month-old rats compared to the young animals. These alterations in the BH4 levels and its synthesizing enzyme kinetics in specific brain areas and adrenal glands of aged rats are consistent with the reported changes in the catecholamine function in central and peripheral nervous system with aging.

Original languageEnglish (US)
Pages (from-to)627-632
Number of pages6
JournalNeurobiology of Aging
Volume16
Issue number4
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

GTP Cyclohydrolase
Adrenal Glands
Brain
Cerebellum
Peripheral Nervous System
Frontal Lobe
Substantia Nigra
Enzymes
sapropterin
Hypothalamus
Catecholamines
Central Nervous System

Keywords

  • Adrenal glands
  • Aging
  • Central nervous system
  • GTP-cyclohydrolase
  • Tetrahydrobiopterin

ASJC Scopus subject areas

  • Aging
  • Developmental Biology
  • Geriatrics and Gerontology
  • Clinical Neurology
  • Neuroscience(all)
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

Cite this

Age-related changes in tetrahydrobiopterin and GTP-cyclohydrolase activity in the brain and adrenal gland of rats. / Hossain, Mir Ahamed; Masserano, Joseph M.; Weiner, Norman.

In: Neurobiology of Aging, Vol. 16, No. 4, 1995, p. 627-632.

Research output: Contribution to journalArticle

Hossain, Mir Ahamed ; Masserano, Joseph M. ; Weiner, Norman. / Age-related changes in tetrahydrobiopterin and GTP-cyclohydrolase activity in the brain and adrenal gland of rats. In: Neurobiology of Aging. 1995 ; Vol. 16, No. 4. pp. 627-632.
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