Age-dependent structural changes in intact human lenses detected by synchrotron radiation x-ray scattering. Correlation with Maillard reaction protein fluorescence

G. Suarez, A. L. Oronsky, M. H.L.J. Koch

Research output: Contribution to journalArticle

Abstract

To understand alterations in the spatial organization of the crystallins, the major determinant of lens transparency, the x-ray scattering by intact normal human lenses from individuals 6-82 years of age was measured using synchrotron radiation. The angular dependence of the integrated scattering intensity is consistent with short-range order within the crystallin assemblies. A significant change in the scattering patterns of the lenses occurs after 55 years of age, in parallel with an increase of the fluorescence of the urea-insoluble crystallin fraction. This correlation suggests a gradual derangement of the short-range order as a result of cross- linking of the crystallin subunits by advanced Maillard reaction products that are generated by the continuous reaction of sugars, such as glucose or fructose, with proteins.

Original languageEnglish (US)
Pages (from-to)17716-17721
Number of pages6
JournalJournal of Biological Chemistry
Volume268
Issue number24
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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