Age-dependent effects of severe traumatic brain injury on cerebral dopaminergic activity in newborn and juvenile pigs

Bernd Walter, Peter Brust, Frank Füchtner, Marco Müller, Rainer Hinz, Hiroto Kuwabara, Harald Fritz, Ulrich Zwiener, Reinhard Bauer

Research output: Contribution to journalArticle

Abstract

There is evidence that the dopaminergic system is sensitive to traumatic brain injury (TBI). However, the age-dependency of this sensitivity has not been studied together with brain oxidative metabolism. We postulate that the acute effects of severe TBI on brain dopamine turnover are age-dependent. Therefore 18F-labelled 6-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) together with Positron-Emission-Tomography (PET) was used to estimate the activity of the aromatic amino acid decarboxylase (AADC) in the brain of 11 newborn piglets (7-10 days old) and nine juvenile pigs (6-7 weeks old). Six newborn and five juvenile animals were subjected to a severe fluid-percussion (FP) induced TBI. The remaining animals were used as sham operated untreated control groups. Simultaneously, the regional cerebral blood flow (CBF) was measured with colored microspheres and the cerebral metabolic rates of oxygen and glucose were determined. At 1 h after FP-TBI, [18F]FDOPA was infused and PET scanning was performed for 2 h. At 2 h after FP-TBI administration, a second series of measurements of physiological values including CBF and brain oxidative metabolism data had been obtained. Severe FP-TBI elicited a marked increase in the rate constant for fluorodopamine production (k3 FDOPA) in all brain regions of newborn piglets studied by between 97% (mesencephalon) and 143% (frontal cortex) (p <0.05). In contrast, brain hemodynamics and cerebral oxidative metabolism remained unaltered after TBI. Furthermore, the permeability-surface area product of FDOPA (PSFDOPA) was unchanged. In addition, regional blood flow differences between corresponding ipsi- and contralateral brain regions did not occur after TBI. Thus, it is suggested that severe FP-TBI induces an upregulation of AADC activity of newborn piglets that is not related to alterations in brain oxidative metabolism.

Original languageEnglish (US)
Pages (from-to)1076-1089
Number of pages14
JournalJournal of Neurotrauma
Volume21
Issue number8
DOIs
StatePublished - Aug 2004

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Swine
Percussion
Brain
Cerebrovascular Circulation
Regional Blood Flow
Positron-Emission Tomography
Aromatic-L-Amino-Acid Decarboxylases
Traumatic Brain Injury
Carboxy-Lyases
Levodopa
Frontal Lobe
Mesencephalon
Microspheres
Permeability
Dopamine
Up-Regulation
Hemodynamics
Oxygen
Amino Acids
Glucose

Keywords

  • Colored microspheres
  • Dopamine metabolism
  • Fluid-percussion
  • Juvenile pig
  • Newbom piglets
  • Pediatric brain injury
  • Positron emission tomography

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Age-dependent effects of severe traumatic brain injury on cerebral dopaminergic activity in newborn and juvenile pigs. / Walter, Bernd; Brust, Peter; Füchtner, Frank; Müller, Marco; Hinz, Rainer; Kuwabara, Hiroto; Fritz, Harald; Zwiener, Ulrich; Bauer, Reinhard.

In: Journal of Neurotrauma, Vol. 21, No. 8, 08.2004, p. 1076-1089.

Research output: Contribution to journalArticle

Walter, B, Brust, P, Füchtner, F, Müller, M, Hinz, R, Kuwabara, H, Fritz, H, Zwiener, U & Bauer, R 2004, 'Age-dependent effects of severe traumatic brain injury on cerebral dopaminergic activity in newborn and juvenile pigs', Journal of Neurotrauma, vol. 21, no. 8, pp. 1076-1089. https://doi.org/10.1089/0897715041651024
Walter, Bernd ; Brust, Peter ; Füchtner, Frank ; Müller, Marco ; Hinz, Rainer ; Kuwabara, Hiroto ; Fritz, Harald ; Zwiener, Ulrich ; Bauer, Reinhard. / Age-dependent effects of severe traumatic brain injury on cerebral dopaminergic activity in newborn and juvenile pigs. In: Journal of Neurotrauma. 2004 ; Vol. 21, No. 8. pp. 1076-1089.
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