Age-associated telomere attrition of lymphocytes in vivo is co-ordinated with changes in telomerase activity, composition of lymphocyte subsets and health conditions

Yun Lin, Amanda Damjanovic, E. Jeffrey Metter, Huy Nguyen, Thai Truong, Kevin Najarro, Christa Morris, Dan L. Longo, Ming Zhan, Luigi Ferrucci, Richard J. Hodes, Nan ping Weng

Research output: Contribution to journalArticle

Abstract

Telomeres are essential in maintaining chromosome integrity and in controlling cellular replication. Attrition of telomere length in peripheral blood mononuclear cells (PBMCs) with age is well documented from cross-sectional studies. But the actual in vivo changes in telomere lengths and its relationship with the contributing factors within the individuals with age have not been fully addressed. In the present paper, we report a longitudinal analysis of telomere length in the PBMCs, lymphocytes and monocytes of 216 human subjects aged from 20-90 years assessed at 0-, 5-and 12-year follow-up. For the 5-and 12-year follow-up, telomere length in the PBMCs decreased in 34% and 46%, exhibited no detectable change in 56% and 47% and increased in 10% and 7% of the subjects respectively. The rate of telomere change was distinct for T-cells, B-cells and monocytes for any given subject. Telomerase activity declined with age in the resting T-cells and B-cells and the activated T-cells. Finally, a significant portion of telomere attrition in T-cells with age was explained by a decline in the telomerase activity, decreased naïve cells and the change in physiological conditions such as elevated blood glucose and interleukin (IL)-6 levels. These findings show that changes in the telomere length of the PBMCs with age in vivo occur at different rates in different individuals and cell types and reveal that changes in the telomere length in the T-cells with age is influenced by the telomerase activity, naïve T-cell percentage and changes in health conditions.

Original languageEnglish (US)
Pages (from-to)367-377
Number of pages11
JournalClinical Science
Volume128
Issue number6
DOIs
StatePublished - 2015
Externally publishedYes

    Fingerprint

Keywords

  • Aging
  • Glucose
  • Interleukin 6 (il-6)
  • Lymphocyte
  • Telomerase
  • Telomere length

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Lin, Y., Damjanovic, A., Jeffrey Metter, E., Nguyen, H., Truong, T., Najarro, K., Morris, C., Longo, D. L., Zhan, M., Ferrucci, L., Hodes, R. J., & Weng, N. P. (2015). Age-associated telomere attrition of lymphocytes in vivo is co-ordinated with changes in telomerase activity, composition of lymphocyte subsets and health conditions. Clinical Science, 128(6), 367-377. https://doi.org/10.1042/CS20140481