Age-associated b cells express a diverse repertoire of vh and vκ genes with somatic hypermutation

Lisa M. Russell Knode, Martin S. Naradikian, Arpita Myles, Jean L. Scholz, Yi Hao, Danya Liu, Mandy L. Ford, John W. Tobias, Michael P. Cancro, Patricia J. Gearhart

Research output: Contribution to journalArticle

Abstract

The origin and nature of age-associated B cells (ABCs) in mice are poorly understood. In this article, we show that their emergence required MHC class II and CD40/CD40L interactions. Young donor B cells were adoptively transferred into congenic recipients and allowed to remain for 1 mo in the absence of external Ag. B cells expressing the T-bet transcription factor, a marker for ABCs, were generated after multiple cell divisions from C57BL/6 donors but not from MHC class II-or CD40-deficient donors. Furthermore, old CD154 (CD40L)-deficient mice did not accrue ABCs, confirming that they arise primarily through T-dependent interactions. To determine what Igs ABCs express, we sequenced VH and Vk rearranged genes from unimmunized 22-mo-old C57BL/6 mice and showed that they had a heterogeneous repertoire, which was comparable to that seen in old follicular and marginal zone B cell subsets. However, in contrast to the follicular and marginal zone cells, ABCs displayed significant somatic hypermutation. The mutation frequency was lower than found in germinal center cells after deliberate immunization, suggesting that ABCs have undergone mild stimulation from endogenous Ags over time. These observations show that quiescent ABCs are Ag-experienced cells that accumulate during T cell-dependent responses to diverse Ags during the life of an individual.

Original languageEnglish (US)
Pages (from-to)1921-1927
Number of pages7
JournalJournal of Immunology
Volume198
Issue number5
DOIs
StatePublished - Mar 1 2017

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B-Lymphocytes
Genes
CD40 Ligand
B-Lymphocyte Subsets
Germinal Center
Mutation Rate
Inbred C57BL Mouse
Cell Division
Immunization
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Russell Knode, L. M., Naradikian, M. S., Myles, A., Scholz, J. L., Hao, Y., Liu, D., ... Gearhart, P. J. (2017). Age-associated b cells express a diverse repertoire of vh and vκ genes with somatic hypermutation. Journal of Immunology, 198(5), 1921-1927. https://doi.org/10.4049/jimmunol.1601106

Age-associated b cells express a diverse repertoire of vh and vκ genes with somatic hypermutation. / Russell Knode, Lisa M.; Naradikian, Martin S.; Myles, Arpita; Scholz, Jean L.; Hao, Yi; Liu, Danya; Ford, Mandy L.; Tobias, John W.; Cancro, Michael P.; Gearhart, Patricia J.

In: Journal of Immunology, Vol. 198, No. 5, 01.03.2017, p. 1921-1927.

Research output: Contribution to journalArticle

Russell Knode, LM, Naradikian, MS, Myles, A, Scholz, JL, Hao, Y, Liu, D, Ford, ML, Tobias, JW, Cancro, MP & Gearhart, PJ 2017, 'Age-associated b cells express a diverse repertoire of vh and vκ genes with somatic hypermutation', Journal of Immunology, vol. 198, no. 5, pp. 1921-1927. https://doi.org/10.4049/jimmunol.1601106
Russell Knode LM, Naradikian MS, Myles A, Scholz JL, Hao Y, Liu D et al. Age-associated b cells express a diverse repertoire of vh and vκ genes with somatic hypermutation. Journal of Immunology. 2017 Mar 1;198(5):1921-1927. https://doi.org/10.4049/jimmunol.1601106
Russell Knode, Lisa M. ; Naradikian, Martin S. ; Myles, Arpita ; Scholz, Jean L. ; Hao, Yi ; Liu, Danya ; Ford, Mandy L. ; Tobias, John W. ; Cancro, Michael P. ; Gearhart, Patricia J. / Age-associated b cells express a diverse repertoire of vh and vκ genes with somatic hypermutation. In: Journal of Immunology. 2017 ; Vol. 198, No. 5. pp. 1921-1927.
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