Age and sun exposure-related widespread genomic blocks of hypomethylation in nonmalignant skin

Amy R. Vandiver, Rafael A. Irizarry, Kasper Daniel Hansen, Luis Garza, Arni Runarsson, Xin Li, Anna Lien-Lun Chien, Timothy S Wang, Sherry G. Leung, Sewon Kang, Andrew P Feinberg

Research output: Contribution to journalArticle

Abstract

Background: Aging and sun exposure are the leading causes of skin cancer. It has been shown that epigenetic changes, such as DNA methylation, are well established mechanisms for cancer, and also have emerging roles in aging and common disease. Here, we directly ask whether DNA methylation is altered following skin aging and/or chronic sun exposure in humans. Results: We compare epidermis and dermis of both sun-protected and sun-exposed skin derived from younger subjects (under 35years old) and older subjects (over 60years old), using the Infinium HumanMethylation450 array and whole genome bisulfite sequencing. We observe large blocks of the genome that are hypomethylated in older, sun-exposed epidermal samples, with the degree of hypomethylation associated with clinical measures of photo-aging. We replicate these findings using whole genome bisulfite sequencing, comparing epidermis from an additional set of younger and older subjects. These blocks largely overlap known hypomethylated blocks in colon cancer and we observe that these same regions are similarly hypomethylated in squamous cell carcinoma samples. Conclusions: These data implicate large scale epigenomic change in mediating the effects of environmental damage with photo-aging.

Original languageEnglish (US)
Article number80
JournalGenome Biology
Volume16
Issue number1
DOIs
StatePublished - Apr 16 2015

Fingerprint

Solar System
photoaging
skin (animal)
bisulfites
skin
genomics
epidermis (animal)
DNA methylation
epigenetics
Skin
genome
cancer
methylation
Genome
DNA Methylation
Epigenomics
Epidermis
dermis
squamous cell carcinoma
colorectal neoplasms

ASJC Scopus subject areas

  • Cell Biology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Age and sun exposure-related widespread genomic blocks of hypomethylation in nonmalignant skin. / Vandiver, Amy R.; Irizarry, Rafael A.; Hansen, Kasper Daniel; Garza, Luis; Runarsson, Arni; Li, Xin; Chien, Anna Lien-Lun; Wang, Timothy S; Leung, Sherry G.; Kang, Sewon; Feinberg, Andrew P.

In: Genome Biology, Vol. 16, No. 1, 80, 16.04.2015.

Research output: Contribution to journalArticle

Vandiver, Amy R. ; Irizarry, Rafael A. ; Hansen, Kasper Daniel ; Garza, Luis ; Runarsson, Arni ; Li, Xin ; Chien, Anna Lien-Lun ; Wang, Timothy S ; Leung, Sherry G. ; Kang, Sewon ; Feinberg, Andrew P. / Age and sun exposure-related widespread genomic blocks of hypomethylation in nonmalignant skin. In: Genome Biology. 2015 ; Vol. 16, No. 1.
@article{d66c002e4cab4aa087d8f6a260a058db,
title = "Age and sun exposure-related widespread genomic blocks of hypomethylation in nonmalignant skin",
abstract = "Background: Aging and sun exposure are the leading causes of skin cancer. It has been shown that epigenetic changes, such as DNA methylation, are well established mechanisms for cancer, and also have emerging roles in aging and common disease. Here, we directly ask whether DNA methylation is altered following skin aging and/or chronic sun exposure in humans. Results: We compare epidermis and dermis of both sun-protected and sun-exposed skin derived from younger subjects (under 35years old) and older subjects (over 60years old), using the Infinium HumanMethylation450 array and whole genome bisulfite sequencing. We observe large blocks of the genome that are hypomethylated in older, sun-exposed epidermal samples, with the degree of hypomethylation associated with clinical measures of photo-aging. We replicate these findings using whole genome bisulfite sequencing, comparing epidermis from an additional set of younger and older subjects. These blocks largely overlap known hypomethylated blocks in colon cancer and we observe that these same regions are similarly hypomethylated in squamous cell carcinoma samples. Conclusions: These data implicate large scale epigenomic change in mediating the effects of environmental damage with photo-aging.",
author = "Vandiver, {Amy R.} and Irizarry, {Rafael A.} and Hansen, {Kasper Daniel} and Luis Garza and Arni Runarsson and Xin Li and Chien, {Anna Lien-Lun} and Wang, {Timothy S} and Leung, {Sherry G.} and Sewon Kang and Feinberg, {Andrew P}",
year = "2015",
month = "4",
day = "16",
doi = "10.1186/s13059-015-0644-y",
language = "English (US)",
volume = "16",
journal = "Genome Biology",
issn = "1474-7596",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Age and sun exposure-related widespread genomic blocks of hypomethylation in nonmalignant skin

AU - Vandiver, Amy R.

AU - Irizarry, Rafael A.

AU - Hansen, Kasper Daniel

AU - Garza, Luis

AU - Runarsson, Arni

AU - Li, Xin

AU - Chien, Anna Lien-Lun

AU - Wang, Timothy S

AU - Leung, Sherry G.

AU - Kang, Sewon

AU - Feinberg, Andrew P

PY - 2015/4/16

Y1 - 2015/4/16

N2 - Background: Aging and sun exposure are the leading causes of skin cancer. It has been shown that epigenetic changes, such as DNA methylation, are well established mechanisms for cancer, and also have emerging roles in aging and common disease. Here, we directly ask whether DNA methylation is altered following skin aging and/or chronic sun exposure in humans. Results: We compare epidermis and dermis of both sun-protected and sun-exposed skin derived from younger subjects (under 35years old) and older subjects (over 60years old), using the Infinium HumanMethylation450 array and whole genome bisulfite sequencing. We observe large blocks of the genome that are hypomethylated in older, sun-exposed epidermal samples, with the degree of hypomethylation associated with clinical measures of photo-aging. We replicate these findings using whole genome bisulfite sequencing, comparing epidermis from an additional set of younger and older subjects. These blocks largely overlap known hypomethylated blocks in colon cancer and we observe that these same regions are similarly hypomethylated in squamous cell carcinoma samples. Conclusions: These data implicate large scale epigenomic change in mediating the effects of environmental damage with photo-aging.

AB - Background: Aging and sun exposure are the leading causes of skin cancer. It has been shown that epigenetic changes, such as DNA methylation, are well established mechanisms for cancer, and also have emerging roles in aging and common disease. Here, we directly ask whether DNA methylation is altered following skin aging and/or chronic sun exposure in humans. Results: We compare epidermis and dermis of both sun-protected and sun-exposed skin derived from younger subjects (under 35years old) and older subjects (over 60years old), using the Infinium HumanMethylation450 array and whole genome bisulfite sequencing. We observe large blocks of the genome that are hypomethylated in older, sun-exposed epidermal samples, with the degree of hypomethylation associated with clinical measures of photo-aging. We replicate these findings using whole genome bisulfite sequencing, comparing epidermis from an additional set of younger and older subjects. These blocks largely overlap known hypomethylated blocks in colon cancer and we observe that these same regions are similarly hypomethylated in squamous cell carcinoma samples. Conclusions: These data implicate large scale epigenomic change in mediating the effects of environmental damage with photo-aging.

UR - http://www.scopus.com/inward/record.url?scp=84939211019&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84939211019&partnerID=8YFLogxK

U2 - 10.1186/s13059-015-0644-y

DO - 10.1186/s13059-015-0644-y

M3 - Article

VL - 16

JO - Genome Biology

JF - Genome Biology

SN - 1474-7596

IS - 1

M1 - 80

ER -