TY - JOUR
T1 - Agaphelin modulates the activation of human bronchial epithelial cells induced by lipopolysaccharide and IL-4
AU - Favarin, Daniely Cornélio
AU - Pereira, Aline Beatriz Mahler
AU - Francischetti, Ivo M.B.
AU - da Silva, Marcos Vinicius
AU - Rodrigues, Virmondes
AU - da Silva, Paulo Roberto
AU - Valenzuela, Jesus G.
AU - Teixeira, David Nascimento Silva
AU - Oliveira, Carlo José Freire
AU - Rogério, Alexandre de Paula
N1 - Funding Information:
This work was supported by Grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (no. 475349/2010-5 ), Fundação de Apoio a Pesquisa do Estado de Minas Gerais (FAPEMIG; APQ 01631/11 e APQ-01873-14 ). Rede de Pesquisa em Doenças Infecciosas Humanas e Animais do Estado de Minas Gerais and Universidade Federal do Triângulo Mineiro (UFTM), Brazil. This study was also financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.
Funding Information:
This work was supported by Grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (no. 475349/2010-5), Fundação de Apoio a Pesquisa do Estado de Minas Gerais (FAPEMIG; APQ 01631/11 e APQ-01873-14). Rede de Pesquisa em Doenças Infecciosas Humanas e Animais do Estado de Minas Gerais and Universidade Federal do Triângulo Mineiro (UFTM), Brazil. This study was also financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.
Publisher Copyright:
© 2020 Elsevier GmbH
PY - 2020/5
Y1 - 2020/5
N2 - Sand fly saliva presents molecules with potential to development of compounds for treatment of inflammatory diseases. Agaphelin, isolated from the saliva of the mosquito Anopheles gambiae, demonstrates anti-inflammatory properties such as neutrophils chemotaxis inhibition. Here, we extend these results and evaluated the role of agaphelin (0.1−100 nM) in an in vitro model consisting in the activation of human bronchial epithelial cells (BEAS-2B) by IL-4 (50 ng/mL) or lipopolysaccharide (LPS; 10 ng/mL). Agaphelin is non-cytotoxic for BEAS-2B cells. Notably, agaphelin markedly reduces CCL2 and IL-8 production induced by IL-4 or LPS, without altering the IL-10 production. The TLR4 expression and STAT1 phosphorylation induced by LPS were inhibited by agaphlin. In addition, agaphelin decreased the phosphorylation of STAT6 induce by IL-4, whose effect was independent of IL-4-binding activity. Taken together, these findings identify agaphelin as a potential anti-inflammatory therapeutic agent for airway inflammations.
AB - Sand fly saliva presents molecules with potential to development of compounds for treatment of inflammatory diseases. Agaphelin, isolated from the saliva of the mosquito Anopheles gambiae, demonstrates anti-inflammatory properties such as neutrophils chemotaxis inhibition. Here, we extend these results and evaluated the role of agaphelin (0.1−100 nM) in an in vitro model consisting in the activation of human bronchial epithelial cells (BEAS-2B) by IL-4 (50 ng/mL) or lipopolysaccharide (LPS; 10 ng/mL). Agaphelin is non-cytotoxic for BEAS-2B cells. Notably, agaphelin markedly reduces CCL2 and IL-8 production induced by IL-4 or LPS, without altering the IL-10 production. The TLR4 expression and STAT1 phosphorylation induced by LPS were inhibited by agaphlin. In addition, agaphelin decreased the phosphorylation of STAT6 induce by IL-4, whose effect was independent of IL-4-binding activity. Taken together, these findings identify agaphelin as a potential anti-inflammatory therapeutic agent for airway inflammations.
KW - Agaphelin
KW - Bronchial epithelial cells
KW - IL-4
KW - LPS
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U2 - 10.1016/j.imbio.2020.151937
DO - 10.1016/j.imbio.2020.151937
M3 - Article
C2 - 32201094
AN - SCOPUS:85081948306
SN - 0171-2985
VL - 225
JO - Immunobiology
JF - Immunobiology
IS - 3
M1 - 151937
ER -