AFM force measurements on microtubule-associated proteins: The projection domain exerts a long-range repulsive force

Rajendrani Mukhopadhyay, Jan H. Hoh

Research output: Contribution to journalArticle

Abstract

Microtubule-associated proteins (MAPs) are thought to control spacing between microtubules. We propose that the projection domain is largely unstructured and exerts a long-range repulsive force that is predominantly entropic in origin, providing a physical mechanism for maintaining spacing. To test this hypothesis, we developed an experimental system where MAPs are electrostatically end-attached to a flat surface, such that the projection domains extend away from the surface. Atomic force microscopy force measurements on this system show that projection domains exert a long-range ( > 100 nm) repulsive force. This force depends on the ionic strength of the solution in a way that is consistent with a polyelectrolyte polymer brush.

Original languageEnglish (US)
Pages (from-to)374-378
Number of pages5
JournalFEBS Letters
Volume505
Issue number3
DOIs
StatePublished - Sep 21 2001

Keywords

  • Entropic exclusion
  • Microtubule-associated protein 2
  • Tau
  • Unstructured

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'AFM force measurements on microtubule-associated proteins: The projection domain exerts a long-range repulsive force'. Together they form a unique fingerprint.

  • Cite this